000135842 001__ 135842
000135842 005__ 20240619140701.0
000135842 0247_ $$2doi$$a10.1016/j.scitotenv.2024.173564
000135842 0248_ $$2sideral$$a138842
000135842 037__ $$aART-2024-138842
000135842 041__ $$aeng
000135842 100__ $$0(orcid)0000-0002-8066-7117$$aBakir, Mariam
000135842 245__ $$aExploring the impact of silver-based nanomaterial feed additives on green algae through single-cell techniques
000135842 260__ $$c2024
000135842 5060_ $$aAccess copy available to the general public$$fUnrestricted
000135842 5203_ $$aSilver in its various forms, including dissolved silver ions (Ag+) and silver nanoparticles (AgNPs), is a promising alternative to traditional antibiotics, largely used in livestock as feed additives and could contribute to the decrease and avoidance of the development of antibiotic resistance. The present study aims to assess the potential ecotoxicity of a silver-based nanomaterial (Ag-kaolin), the feed supplemented with the nanomaterial and the faeces since the latter are the ones that finally reach the environment. To this end, green alga Raphidocellis subcapitata was exposed to the extracts of Ag-kaolin, supplemented feed, and pig faeces for 72 h, along with Ag+ and AgNPs as controls for comparison purposes. Given the complexity of the studied materials, single-cell techniques were used to follow the changes in the cell numbers and chlorophyll fluorescence by flow cytometry, and the accumulation of silver in the exposed cells by single cell inductively coupled plasma mass spectrometry (SC-ICP-MS). Changes in cell morphology were observed by cell imaging multimode reader. The results revealed a decrease in chlorophyll fluorescence, even at low concentrations of Ag-kaolin (10 μg L−1) after 48 h of exposure. Additionally, complete growth inhibition was found with this material like the results obtained by exposure to Ag+. For the supplemented feed, a concentration of 50 μg L−1 was necessary to achieve complete growth inhibition. However, the behaviour differed for the leachate of faeces, which released Ag2S and AgCl alongside Ag+ and AgNPs. At 50 μg L−1, inhibition was minimal, primarily due to the predominance of less toxic Ag2S in the leachate. The uptake of silver by the cells was confirmed with all the samples through SC-ICP-MS analysis. These findings demonstrate that the use of Ag-kaolin as a feed supplement will lead to a low environmental impact.
000135842 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E29-20R$$9info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/OUTBIOTICS-EFA183/16$$9info:eu-repo/grantAgreement/ES/MCIU-FEDER/RTI2018-096111-B-I00$$9info:eu-repo/grantAgreement/ES/UZ-IBERCAJA-CAI/CB11-22
000135842 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000135842 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000135842 700__ $$0(orcid)0000-0002-8460-6020$$aJiménez, María S.$$uUniversidad de Zaragoza
000135842 700__ $$0(orcid)0000-0002-4169-0357$$aLaborda, Francisco$$uUniversidad de Zaragoza
000135842 700__ $$aSlaveykova, Vera I.
000135842 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000135842 773__ $$g939 (2024), 173564 [9 pp.]$$pSci. total environ.$$tSCIENCE OF THE TOTAL ENVIRONMENT$$x0048-9697
000135842 8564_ $$s4007226$$uhttps://zaguan.unizar.es/record/135842/files/texto_completo.pdf$$yVersión publicada
000135842 8564_ $$s1904436$$uhttps://zaguan.unizar.es/record/135842/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000135842 909CO $$ooai:zaguan.unizar.es:135842$$particulos$$pdriver
000135842 951__ $$a2024-06-19-13:22:54
000135842 980__ $$aARTICLE