135891 20250203110524.0 doi 10.1016/j.ifset.2018.04.012 sideral 106425 ART-2018-106425 eng Martínez, J.M. Universidad de Zaragoza (orcid)0000-0002-9337-417X Pulsed electric field-assisted extraction of carotenoids from fresh biomass of Rhodotorula glutinis 2018 The aim of this study was to demonstrate the potential of PEF for inducing autolysis of R. glutinis, with the purpose of designing a more efficient and ecofriendly carotenoid extraction process: an extraction from fresh biomass, using cheaper, non-toxic, environmental-friendly solvents. Propidium iodide uptake and release of intracellular components revealed the irreversible electroporation of R. glutinis by PEF. Flow cytometry measurements detected morphological changes in PEF-treated R. glutinis cells during incubation caused by the autolysis triggering effect of electroporation. After submitting the fresh biomass to a PEF treatment (15 kV/cm, 150 µs) that irreversibly electroporated more than the 90% of the cells, ethanol proved ineffective for extracting carotenoids from fresh biomass of R. glutinis. However, after incubating the PEF-treated fresh biomass for 24 h at 20 °C in a pH 7 buffer, ca. 240 µg/g d.w. of carotenoids were recovered after 1 h of extraction in ethanol. The highest amount of carotenoids extracted (375 µg/g d.w.) from the PEF-treated cells of R. glutinis was obtained after having incubated them at 25 °C for 24 h in a medium of pH 8.0. The improvement in carotenoid extraction by incubating the R. glutinis cells after PEF treatment seems to be caused by PEF-triggered autolysis, which tends to disrupt the association of carotenoids with other molecules present in the cytoplasm, and causes a degradation of the cell wall. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.085 2018 FOOD SCIENCE & TECHNOLOGY 14 / 135 = 0.104 2018 Q1 T1 1.43 2018 Chemistry (miscellaneous) 2018 Q1 Industrial and Manufacturing Engineering 2018 Q1 Food Science 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Delso, C. Universidad de Zaragoza (orcid)0000-0003-4744-8649 Angulo, J. Álvarez, I. Universidad de Zaragoza (orcid)0000-0003-2430-858X Raso, J. Universidad de Zaragoza (orcid)0000-0003-3957-9091 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 47 (2018), 421-427 Innov. food sci. emerg. technol. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES 1466-8564 897281 http://zaguan.unizar.es/record/135891/files/texto_completo.pdf Versión publicada 2648151 http://zaguan.unizar.es/record/135891/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:135891 articulos driver 2025-02-03-11:02:11 ARTICLE