doi:10.1007/s00216-024-05323-8engBen-Jeddou, KhaoulaBakir, MariamJiménez, María S.Gómez, María T.Abad-Álvaro, IsabelLaborda, FranciscoNanosilver-based materials as feed additives: Evaluation of their transformations along in vitro gastrointestinal digestion in pigs and chickens by using an ICP-MS based analytical platformART-2024-138893The use of a new nanomaterial in the feed chain requires a risk assessment that involves in vitro gastrointestinal digestions to predict its degradation and oral exposure to nanoparticles. In this study, a nanosilver-based material was incorporated into pig and chicken feed as a growth-promoting additive and subjected to the corresponding in vitro gastrointestinal digestions. An inductively coupled plasma mass spectroscopy (ICP-MS) analytical platform was used to obtain information about the silver released in the different digestion phases. It included conventional ICP-MS for total silver determination, but also single particle ICP-MS and coupling to hydrodynamic chromatography for detection of dissolved and particulate silver. The bioaccessible fraction in the intestinal phase accounted for 8–13% of the total silver, mainly in the form of dissolved Ag(I) species, with less than 0.1% as silver-containing particles. Despite the additive behaving differently in pig and chicken digestions, the feed matrix played a relevant role in the fate of the silver.2024http://zaguan.unizar.es/record/13591910.1007/s00216-024-05323-8http://zaguan.unizar.es/record/135919oai:zaguan.unizar.es:135919info:eu-repo/grantAgreement/ES/DGA-FEDER/E29-23Rinfo:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalentThis project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801586-IberusTalentinfo:eu-repo/grantAgreement/ES/MICINN/PID2021-123203OB-I00info:eu-repo/grantAgreement/ES/NextGenerationEU/MZ-240621Analytical and Bioanalytical Chemistry 416 (2024), 3821-3833byhttps://creativecommons.org/licenses/by/4.0/deed.esinfo:eu-repo/semantics/openAccess