000135972 001__ 135972
000135972 005__ 20250923084429.0
000135972 0247_ $$2doi$$a10.3390/app14104016
000135972 0248_ $$2sideral$$a138969
000135972 037__ $$aART-2024-138969
000135972 041__ $$aeng
000135972 100__ $$aGarin-Murguialday, Nerea
000135972 245__ $$aLactic acid bacteria and bacillus subtilis as potential protective cultures for biopreservation in the food industry
000135972 260__ $$c2024
000135972 5060_ $$aAccess copy available to the general public$$fUnrestricted
000135972 5203_ $$aThe use of bacteria and/or their compounds is an alternative to the use of positive-list additives that the food industry is using as a tool to meet consumer demands for more natural, long-shelf-life, and healthy products, in short, to offer clean label foods. The aim of this study is to investigate the suitability of cell-free supernatants (CFSs) from Qualified Presumption of Safety strains as bioprotective cultures. Out of an initial screening panel of about 200 isolates, strains Pediococcus acidilactici CNTA 1059, Lactiplantibacillus plantarum CNTA 600, Levilactobacillus brevis CNTA 1374, and Bacillus subtilis CNTA 517 demonstrated strong antimicrobial activity against, especially, Gram-positive bacteria. The CFSs of these four strains showed minimum inhibitory concentration values between 0.15% and 5% against Listeria monocytogenes and Lentilactobacillus parabuchneri. None of the four selected strains exhibited acquired resistance to target antibiotics, and the non-toxigenicity of all the CFSs was demonstrated. In the case of the three lactic acid bacteria, the presence of bacteriocin-like inhibitory substances was confirmed following the decline in antimicrobial activity due to treatment with proteases. Regarding B. subtilis, biosynthetic gene clusters for different bacteriocin-like substances, including protease-resistant lipoproteins, were found via whole-genome sequencing. In addition, all of the CFSs exhibited stable antimicrobial activity at a wide range of temperatures (70–121 °C) used for the pasteurization and sterilization of food products, with a loss of antimicrobial activity ranging from 3% to 28%. These results point to the possibility that CFSs from these strains could be used in the food industry as a biocontrol tool to develop new products.
000135972 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-123404NB-I00$$9info:eu-repo/grantAgreement/ES/DGA/A06-23R
000135972 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000135972 590__ $$a2.5$$b2024
000135972 592__ $$a0.521$$b2024
000135972 591__ $$aENGINEERING, MULTIDISCIPLINARY$$b50 / 175 = 0.286$$c2024$$dQ2$$eT1
000135972 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b123 / 239 = 0.515$$c2024$$dQ3$$eT2
000135972 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b283 / 460 = 0.615$$c2024$$dQ3$$eT2
000135972 591__ $$aPHYSICS, APPLIED$$b101 / 187 = 0.54$$c2024$$dQ3$$eT2
000135972 593__ $$aEngineering (miscellaneous)$$c2024$$dQ2
000135972 593__ $$aComputer Science Applications$$c2024$$dQ2
000135972 593__ $$aProcess Chemistry and Technology$$c2024$$dQ2
000135972 593__ $$aInstrumentation$$c2024$$dQ2
000135972 593__ $$aMaterials Science (miscellaneous)$$c2024$$dQ2
000135972 593__ $$aFluid Flow and Transfer Processes$$c2024$$dQ2
000135972 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000135972 700__ $$0(orcid)0000-0002-3170-9755$$aEspina, Laura
000135972 700__ $$aVirto, Raquel
000135972 700__ $$0(orcid)0000-0002-0238-6328$$aPagán, Rafael$$uUniversidad de Zaragoza
000135972 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000135972 773__ $$g14, 10 (2024), 4016 [18 pp.]$$pAppl. sci.$$tApplied Sciences (Switzerland)$$x2076-3417
000135972 8564_ $$s545433$$uhttps://zaguan.unizar.es/record/135972/files/texto_completo.pdf$$yVersión publicada
000135972 8564_ $$s2831869$$uhttps://zaguan.unizar.es/record/135972/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000135972 909CO $$ooai:zaguan.unizar.es:135972$$particulos$$pdriver
000135972 951__ $$a2025-09-22-14:42:07
000135972 980__ $$aARTICLE