000136012 001__ 136012
000136012 005__ 20240704095850.0
000136012 0247_ $$2doi$$a10.1016/j.talanta.2024.126417
000136012 0248_ $$2sideral$$a139010
000136012 037__ $$aART-2024-139010
000136012 041__ $$aeng
000136012 100__ $$aMoreira Freire, Bruna
000136012 245__ $$aTracing isotopically labeled selenium nanoparticles in plants via single-particle ICP-mass spectrometry
000136012 260__ $$c2024
000136012 5060_ $$aAccess copy available to the general public$$fUnrestricted
000136012 5203_ $$aAgronomic biofortification using selenium nanoparticles (SeNPs) shows potential for addressing selenium deficiency but further research on SeNPs-plants interaction is required before it can be effectively used to improve nutritional quality. In this work, single-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) was used for tracing isotopically labeled SeNPs (82SeNPs) in Oryza sativa L. tissues. For this purpose, SeNPs with natural isotopic abundance and 82SeNPs were synthesized by a chemical method. The NPs characterization by transmission electron microscopy (TEM) confirmed that enriched NPs maintained the basic properties of unlabeled NPs, showing spherical shape, monodispersity, and sizes in the nano-range (82.8 ± 6.6 nm and 73.2 ± 4.4 nm for SeNPs and 82SeNPs, respectively). The use of 82SeNPs resulted in an 11-fold enhancement in the detection power for ICP-MS analysis, accompanied by an improvement in the signal-to-background ratio and a reduction of the size limits of detection from 89.9 to 39.9 nm in SP-ICP-MS analysis. This enabled 82SeNPs to be tracked in O. sativa L. plants cultivated under foliar application of 82SeNPs. Tracing studies combining SP-ICP-MS and TEM-energy-dispersive X-ray spectroscopy data confirmed the uptake of intact 82SeNPs by rice leaves, with most NPs remaining in the leaves and very few particles translocated to shoots and roots. Translocation of Se from leaves to roots and shoots was found to be lower when applied as NPs compared to selenite application. From the size distributions, as obtained by SP-ICP-MS, it can be concluded that a fraction of the 82SeNPs remained within the same size range as that of the applied NP suspension, while other fraction underwent an agglomeration process in the leaves, as confirmed by TEM images. This illustrates the potential of SP-ICP-MS analysis of isotopically enriched 82SeNPs for tracing NPs in the presence of background elements within complex plant matrices, providing important information about the uptake, accumulation, and biotransformation of SeNPs in rice plants.
000136012 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E43-20R$$9info:eu-repo/grantAgreement/EC/H2020/101034288/EU/International Fellowship Programme for Talent Attraction to the Campus of International Excellence Campus Iberus/IberusExperience$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101034288-IberusExperience$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-122455NB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2021-031093-I
000136012 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000136012 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000136012 700__ $$0(orcid)0000-0001-9582-6283$$aRua-Ibarz, Ana$$uUniversidad de Zaragoza
000136012 700__ $$0(orcid)0000-0001-7087-9901$$aVenâncio Nakadi, Flávio$$uUniversidad de Zaragoza
000136012 700__ $$0(orcid)0000-0002-1856-2058$$aBolea-Fernández, Eduardo$$uUniversidad de Zaragoza
000136012 700__ $$0(orcid)0000-0003-2980-5454$$aBarriuso-Vargas, Juan J.$$uUniversidad de Zaragoza
000136012 700__ $$aLange, Camila Neves
000136012 700__ $$0(orcid)0000-0002-3916-9992$$aAramendía, Maite$$uUniversidad de Zaragoza
000136012 700__ $$aBatista, Bruno Lemos
000136012 700__ $$0(orcid)0000-0002-7450-8769$$aResano, Martín$$uUniversidad de Zaragoza
000136012 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000136012 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000136012 773__ $$g277 (2024), 126417 [11 pp.]$$pTalanta$$tTalanta$$x0039-9140
000136012 8564_ $$s4690444$$uhttps://zaguan.unizar.es/record/136012/files/texto_completo.pdf$$yVersión publicada
000136012 8564_ $$s2421783$$uhttps://zaguan.unizar.es/record/136012/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000136012 909CO $$ooai:zaguan.unizar.es:136012$$particulos$$pdriver
000136012 951__ $$a2024-07-04-08:00:08
000136012 980__ $$aARTICLE