136012 20260217205609.0 doi 10.1016/j.talanta.2024.126417 sideral 139010 ART-2024-139010 eng Moreira Freire, Bruna Tracing isotopically labeled selenium nanoparticles in plants via single-particle ICP-mass spectrometry 2024 Access copy available to the general public Unrestricted Agronomic biofortification using selenium nanoparticles (SeNPs) shows potential for addressing selenium deficiency but further research on SeNPs-plants interaction is required before it can be effectively used to improve nutritional quality. In this work, single-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) was used for tracing isotopically labeled SeNPs (82SeNPs) in Oryza sativa L. tissues. For this purpose, SeNPs with natural isotopic abundance and 82SeNPs were synthesized by a chemical method. The NPs characterization by transmission electron microscopy (TEM) confirmed that enriched NPs maintained the basic properties of unlabeled NPs, showing spherical shape, monodispersity, and sizes in the nano-range (82.8 ± 6.6 nm and 73.2 ± 4.4 nm for SeNPs and 82SeNPs, respectively). The use of 82SeNPs resulted in an 11-fold enhancement in the detection power for ICP-MS analysis, accompanied by an improvement in the signal-to-background ratio and a reduction of the size limits of detection from 89.9 to 39.9 nm in SP-ICP-MS analysis. This enabled 82SeNPs to be tracked in O. sativa L. plants cultivated under foliar application of 82SeNPs. Tracing studies combining SP-ICP-MS and TEM-energy-dispersive X-ray spectroscopy data confirmed the uptake of intact 82SeNPs by rice leaves, with most NPs remaining in the leaves and very few particles translocated to shoots and roots. Translocation of Se from leaves to roots and shoots was found to be lower when applied as NPs compared to selenite application. From the size distributions, as obtained by SP-ICP-MS, it can be concluded that a fraction of the 82SeNPs remained within the same size range as that of the applied NP suspension, while other fraction underwent an agglomeration process in the leaves, as confirmed by TEM images. This illustrates the potential of SP-ICP-MS analysis of isotopically enriched 82SeNPs for tracing NPs in the presence of background elements within complex plant matrices, providing important information about the uptake, accumulation, and biotransformation of SeNPs in rice plants. info:eu-repo/grantAgreement/ES/DGA/E43-20R info:eu-repo/grantAgreement/EC/H2020/101034288/EU/International Fellowship Programme for Talent Attraction to the Campus of International Excellence Campus Iberus/IberusExperience This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101034288-IberusExperience info:eu-repo/grantAgreement/ES/MICINN/PID2021-122455NB-I00 info:eu-repo/grantAgreement/ES/MICINN/RYC2021-031093-I info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 6.1 2024 CHEMISTRY, ANALYTICAL 14 / 111 = 0.126 2024 Q1 T1 0.976 2024 Analytical Chemistry 2024 Q1 Spectroscopy 2024 Q1 Biochemistry 2024 Q2 11.0 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Rua-Ibarz, Ana Universidad de Zaragoza (orcid)0000-0001-9582-6283 Venâncio Nakadi, Flávio Universidad de Zaragoza (orcid)0000-0001-7087-9901 Bolea-Fernández, Eduardo Universidad de Zaragoza (orcid)0000-0002-1856-2058 Barriuso-Vargas, Juan J. Universidad de Zaragoza (orcid)0000-0003-2980-5454 Lange, Camila Neves Aramendía, Maite Universidad de Zaragoza (orcid)0000-0002-3916-9992 Batista, Bruno Lemos Resano, Martín Universidad de Zaragoza (orcid)0000-0002-7450-8769 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 5011 705 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Área Producción Vegetal 277 (2024), 126417 [11 pp.] Talanta Talanta 0039-9140 4690444 http://zaguan.unizar.es/record/136012/files/texto_completo.pdf Versión publicada 2421783 http://zaguan.unizar.es/record/136012/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:136012 articulos driver 2026-02-17-20:48:28 ARTICLE