000136084 001__ 136084
000136084 005__ 20240711103551.0
000136084 0247_ $$2doi$$a10.3390/microorganisms12061084
000136084 0248_ $$2sideral$$a139054
000136084 037__ $$aART-2024-139054
000136084 041__ $$aeng
000136084 100__ $$0(orcid)0000-0002-3485-0682$$aMatovelle, C.
000136084 245__ $$aSubtype distribution of Blastocystis spp. in patients with gastrointestinal symptoms in northern Spain
000136084 260__ $$c2024
000136084 5060_ $$aAccess copy available to the general public$$fUnrestricted
000136084 5203_ $$aLimited molecular data exist on the prevalence and subtype distribution of Blastocystis spp., the most prevalent parasite in human and animal feces worldwide. A total of 44 different subtypes (STs) of Blastocystis are currently recognized based on the sequence of the small subunit ribosomal RNA (SSU-rRNA) gene. This is a molecular study of Blastocystis spp. in hospitalized patients with gastrointestinal symptoms in northern Spain. We analyzed 173 Blastocystis-positive patients with gastrointestinal symptoms by using nested PCR for molecular detection, subtype identification, phylogenetic analyses, and genetic diversity assessment. ST2 (34.1%) and ST3 (34.7%) predominated, followed by ST1 (15.6%) and ST4 (15.6%). Mixed infections with different subtypes were observed in some patients. Sequence analysis revealed for the first time in European humans the allele 88 (a variant of ST1). In other cases, alleles commonly found in animal samples were detected (allele 9 in ST2, allele 34 in ST3, and allele 42 in ST4). Phylogenetic analysis showed high variability in ST1 and ST2, suggesting a polyphyletic origin, while both ST3 and ST4 exhibited higher genetic homogeneity, indicating a possible monophyletic origin and recent transmission to humans. These data confirm Blastocystis spp. subtype diversity and may help in understanding the evolutionary processes and potential zoonotic transmission of this parasite.
000136084 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A16-23R$$9info:eu-repo/grantAgreement/ES/DGA/B43-23R
000136084 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000136084 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000136084 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, J.$$uUniversidad de Zaragoza
000136084 700__ $$0(orcid)0000-0001-5026-5144$$aTejedor, M. T.$$uUniversidad de Zaragoza
000136084 700__ $$0(orcid)0000-0002-3764-0189$$aBeltrán, A.$$uUniversidad de Zaragoza
000136084 700__ $$0(orcid)0000-0002-9981-9045$$aChueca, P.$$uUniversidad de Zaragoza
000136084 700__ $$0(orcid)0000-0003-0210-2919$$aMonteagudo, L.V$$uUniversidad de Zaragoza
000136084 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000136084 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000136084 7102_ $$11001$$2X$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Técnica. Lab. y Talleres
000136084 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000136084 773__ $$g12, 1084 (2024), 15$$pMicroorganisms$$tMicroorganisms$$x2076-2607
000136084 8564_ $$s941166$$uhttps://zaguan.unizar.es/record/136084/files/texto_completo.pdf$$yVersión publicada
000136084 8564_ $$s2729745$$uhttps://zaguan.unizar.es/record/136084/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000136084 909CO $$ooai:zaguan.unizar.es:136084$$particulos$$pdriver
000136084 951__ $$a2024-07-11-08:37:55
000136084 980__ $$aARTICLE