000136147 001__ 136147
000136147 005__ 20240719195437.0
000136147 0247_ $$2doi$$a10.3390/insects15060377
000136147 0248_ $$2sideral$$a139153
000136147 037__ $$aART-2024-139153
000136147 041__ $$aeng
000136147 100__ $$0(orcid)0000-0001-5316-1703$$aYániz, Jesús$$uUniversidad de Zaragoza
000136147 245__ $$aMicrobiota analysis of ejaculated honey bee drone semen and the effect of semen collection method on bacterial loads
000136147 260__ $$c2024
000136147 5060_ $$aAccess copy available to the general public$$fUnrestricted
000136147 5203_ $$aArtificial insemination in queen honey bees is the only tool that provides complete control over mating for research and breeding purposes, making it essential in genetic improvement and conservation programs in this species. The aims of this study were to characterize drone semen bacterial loads by culture-dependent and independent methods and to describe their variation depending on the method of semen collection, the colony and the apiary. In the first experiment, the bacterial loads of semen collected from the seminal vesicles or from ejaculates was studied using culture-dependent methods. The collection method had a significant influence on the overall bacterial count in semen. Out of the 42 semen samples analyzed, 26 (61.9%) tested positive for bacterial isolation. This encompassed the entirety of samples obtained from the seminal vesicles (21 of 21), whereas only 23.8% of those derived from ejaculates (5 out of 21) showed bacterial isolation. In the second experiment, next-generation sequencing techniques were used to describe the microbiome of ejaculated drone semen for the first time. The most abundant phyla were Proteobacteria, Firmicutes, Bacteroidota and Actinobacteriota, while the most abundant genera were Lactobacillus, Staphylococcus, Prevotella, Alloprevotella and Streptococcus. The results showed that the apiary had a significant effect on the community structure composition and abundance of the seminal microbiota, and significative differences in abundance were observed for the genera Sphingomonas, Methylobacterium-Methylorubrum, Bifidobacterium and Alloprevotella. Significant differences were also observed in the richness of the microbiota between apiaries and colonies.
000136147 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2020-112673RB-I00$$9info:eu-repo/grantAgreement/ES/DGA-FSE/A07_23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-119462RA-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2021-032245-I
000136147 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000136147 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000136147 700__ $$aToquet, Marion
000136147 700__ $$0(orcid)0000-0001-8991-325X$$aSantolaria, Pilar$$uUniversidad de Zaragoza
000136147 700__ $$aSilvestre, Miguel Ángel
000136147 700__ $$aToledo-Perona, Raquel
000136147 700__ $$aGómez-Martín, Ángel
000136147 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000136147 773__ $$g15, 377 (2024), 1-11$$pInsects$$tInsects$$x2075-4450
000136147 8564_ $$s1941638$$uhttps://zaguan.unizar.es/record/136147/files/texto_completo.pdf$$yVersión publicada
000136147 8564_ $$s2649791$$uhttps://zaguan.unizar.es/record/136147/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000136147 909CO $$ooai:zaguan.unizar.es:136147$$particulos$$pdriver
000136147 951__ $$a2024-07-19-18:28:09
000136147 980__ $$aARTICLE