000136206 001__ 136206
000136206 005__ 20250923084423.0
000136206 0247_ $$2doi$$a10.3390/ani14121796
000136206 0248_ $$2sideral$$a139111
000136206 037__ $$aART-2024-139111
000136206 041__ $$aeng
000136206 100__ $$aRoca-Geronès, Xavier
000136206 245__ $$aGenetic Variability in Leishmaniasis-Causing Leishmania infantum in Humans and Dogs from North-East Spain
000136206 260__ $$c2024
000136206 5060_ $$aAccess copy available to the general public$$fUnrestricted
000136206 5203_ $$aLeishmania infantum is the primary cause of visceral and cutaneous leishmaniasis in the European Mediterranean region. Subspecies-level characterization of L. infantum aids epidemiological studies by offering insights into the evolution and geographical distribution of the parasite and reservoir identity. In this study, conducted in north-east Spain, 26 DNA samples of L. infantum were analyzed, comprising 21 from 10 humans and 5 from 5 dogs. Minicircle kinetoplast DNA (kDNA) polymerase chain reaction assays using primers MC1 and MC2, followed by sequencing, were employed to assess intraspecific genetic variability. Single-nucleotide polymorphism (SNP) analysis detected seven genotypes (G1, G2, G12*–G15*, and G17*), with five being reported for the first time (*). The most prevalent was the newly described G13 (54%), while the other currently identified genotypes were predominantly found in single samples. The in silico restriction fragment length polymorphism (RFLP) method revealed five genotypes (B, F, N, P, and W), one of them previously unreported (W). Genotype B was the most prevalent (85%), comprising three SNP genotypes (G1, G2, and G13), whereas the other RFLP genotypes were associated with single SNP genotypes. These kDNA genotyping methods revealed significant intraspecific genetic diversity in L. infantum, demonstrating their suitability for fingerprinting and strain monitoring.
000136206 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000136206 590__ $$a2.7$$b2024
000136206 592__ $$a0.733$$b2024
000136206 591__ $$aVETERINARY SCIENCES$$b21 / 170 = 0.124$$c2024$$dQ1$$eT1
000136206 593__ $$aVeterinary (miscellaneous)$$c2024$$dQ1
000136206 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b15 / 86 = 0.174$$c2024$$dQ1$$eT1
000136206 593__ $$aAnimal Science and Zoology$$c2024$$dQ1
000136206 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000136206 700__ $$aSala, Clara
000136206 700__ $$aMarteles, Diana$$uUniversidad de Zaragoza
000136206 700__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza
000136206 700__ $$aRiera, Cristina
000136206 700__ $$aAlcover, Mª Magdalena
000136206 700__ $$aFisa, Roser
000136206 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000136206 773__ $$g14, 12 (2024), 12 pp.$$pAnimals (Basel)$$tAnimals$$x2076-2615
000136206 8564_ $$s1030409$$uhttps://zaguan.unizar.es/record/136206/files/texto_completo.pdf$$yVersión publicada
000136206 8564_ $$s2594652$$uhttps://zaguan.unizar.es/record/136206/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000136206 909CO $$ooai:zaguan.unizar.es:136206$$particulos$$pdriver
000136206 951__ $$a2025-09-22-14:37:45
000136206 980__ $$aARTICLE