000136250 001__ 136250
000136250 005__ 20240731105611.0
000136250 0247_ $$2doi$$a10.1016/j.tice.2021.101589
000136250 0248_ $$2sideral$$a126209
000136250 037__ $$aART-2021-126209
000136250 041__ $$aeng
000136250 100__ $$aCastro A.N.C.
000136250 245__ $$aPatterns of proliferation and cell differentiation during hepatic ontogeny in the alpaca
000136250 260__ $$c2021
000136250 5203_ $$aThe liver has multiple functions that change throughout ontogeny. South American camelids (SAC) have unique characteristics related to adaptation to extreme environments and metabolism. However, the process of hepatic cell differentiation has not been studied in any SAC. We study the patterns of cell differentiation and proliferation in the liver of the alpaca at different times of the ontogeny, excluding the hematopoietic components. Immunohistochemical techniques were performed in 66 specimens, including embryos, fetuses, neonates and adults. Supplementary analyses were performed by lectinhistochemistry. The hepatocytic differentiation was performed by the identification of Hepatocyte (Clone: ¿¿OCH1ES Dako®). It began in the specimens of 1.8-2.5 cm of crown to rump length (CRL), from Days 25–29 (ovulation = Day 0), continued during gestation and intensified towards its end. The cholangiocytic differentiation was performed by the identification of cytokeratin 7 (CK7, Dako®). It was manifested at the final of gestation (specimens of 28.4 cm CRL, from Day 223 onwards). Parenchymal cells underwent a process of gradual differentiation (differentiation of hepatocytes preceded that of cholangiocytes). Cell proliferation was observed along gestation using the nuclear proliferation antigen (PCNA) and Ki-67. Hepatic organogenesis in the alpacas shares similar differentiation and proliferation mechanisms with other altricial, but phylogenetically distant, species. © 2021
000136250 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000136250 590__ $$a1.994$$b2021
000136250 591__ $$aMICROBIOLOGY$$b124 / 137 = 0.905$$c2021$$dQ4$$eT3
000136250 591__ $$aINFECTIOUS DISEASES$$b82 / 95 = 0.863$$c2021$$dQ4$$eT3
000136250 592__ $$a0.277$$b2021
000136250 593__ $$aMicrobiology (medical)$$c2021$$dQ4
000136250 594__ $$a2.0$$b2021
000136250 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000136250 700__ $$aDíaz M.C.
000136250 700__ $$aMendoza Torres G.J.
000136250 700__ $$0(orcid)0000-0001-6629-6842$$aMoreno Burgos B.$$uUniversidad de Zaragoza
000136250 700__ $$aZanuzzi C.
000136250 700__ $$aIllia M.C.
000136250 700__ $$aLendez P.A.
000136250 700__ $$aCarril J.
000136250 700__ $$aGhezzi M.D.
000136250 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola Diez J.J.$$uUniversidad de Zaragoza
000136250 700__ $$aBarbeito C.G.
000136250 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000136250 773__ $$g71 (2021), 101589$$pEnferm. infecc. microbiol. clín.$$tEnfermedades Infecciosas y Microbiologia Clinica$$x0213-005X
000136250 8564_ $$s8816717$$uhttps://zaguan.unizar.es/record/136250/files/texto_completo.pdf$$yVersión publicada
000136250 8564_ $$s2361260$$uhttps://zaguan.unizar.es/record/136250/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000136250 909CO $$ooai:zaguan.unizar.es:136250$$particulos$$pdriver
000136250 951__ $$a2024-07-31-09:21:33
000136250 980__ $$aARTICLE