000136279 001__ 136279
000136279 005__ 20250923084425.0
000136279 0247_ $$2doi$$a10.3390/nano14131149
000136279 0248_ $$2sideral$$a139262
000136279 037__ $$aART-2024-139262
000136279 041__ $$aeng
000136279 100__ $$0(orcid)0000-0003-2672-5790$$aBaselga, Marta
000136279 245__ $$aMelanin-based nanoparticles for lymph node tattooing: experimental, histopathological and ultrastructural study
000136279 260__ $$c2024
000136279 5060_ $$aAccess copy available to the general public$$fUnrestricted
000136279 5203_ $$aIn breast cancer, Targeted Axillary Dissection (TAD) allows for the selective excision of the sentinel lymph node (SLN) during primary tumor surgery. TAD consists of the resection of labelled SLNs prior to neoadjuvant chemotherapy (NACT). Numerous clinical and preclinical studies have explored the use of carbon-based colloids for SLN tattooing prior to NACT. However, carbon vectors show varying degrees of inflammatory reactions and, in about one fifth of cases, carbon particles migrate via the lymphatic pathway to other nodes, causing the SLN to mismatch the tattooed node. To overcome these limitations, in this study, we explored the use of melanin as a staining endogenous pigment. We synthesized and characterized melanin-loaded polymeric nanoparticles (Mel-NPs) and used them to tattoo lymph nodes in pig animal models given the similarity in the size of the human and pig nodes. Mel-NPs tattooed lymph nodes showed high identification rates, reaching 83.3% positive identification 16 weeks after tattooing. We did not observe any reduction in the identification as time increased, implying that the colloid is stable in the lymph node tissue. In addition, we performed histological and ultrastructural studies to characterize the biological behavior of the tag. We observed foreign-body-like granulomatous inflammatory responses associated with Mel-NPs, characterized by the formation of multinucleated giant cells. In addition, electron microscopy studies showed that uptake is mainly performed by macrophages, and that macrophages undergo cellular damage associated with particle uptake.
000136279 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/DTS2019-00131$$9info:eu-repo/grantAgreement/ES/ISCIII/MS19-00092
000136279 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000136279 590__ $$a4.3$$b2024
000136279 592__ $$a0.811$$b2024
000136279 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b177 / 460 = 0.385$$c2024$$dQ2$$eT2
000136279 593__ $$aChemical Engineering (miscellaneous)$$c2024$$dQ1
000136279 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b69 / 147 = 0.469$$c2024$$dQ2$$eT2
000136279 593__ $$aMaterials Science (miscellaneous)$$c2024$$dQ2
000136279 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b81 / 239 = 0.339$$c2024$$dQ2$$eT2
000136279 591__ $$aPHYSICS, APPLIED$$b57 / 187 = 0.305$$c2024$$dQ2$$eT1
000136279 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000136279 700__ $$0(orcid)0000-0002-4437-2581$$aGüemes, Antonio$$uUniversidad de Zaragoza
000136279 700__ $$0(orcid)0000-0002-1505-498X$$aYus, Cristina$$uUniversidad de Zaragoza
000136279 700__ $$aAlejo, Teresa
000136279 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, Víctor$$uUniversidad de Zaragoza
000136279 700__ $$0(orcid)0000-0002-3592-820X$$aArribas, Dolores$$uUniversidad de Zaragoza
000136279 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia
000136279 700__ $$0(orcid)0000-0002-7453-1766$$aMonleón, Eva$$uUniversidad de Zaragoza
000136279 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000136279 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000136279 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000136279 7102_ $$11013$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Cirugía
000136279 773__ $$g14, 13 (2024), 1149 [16 pp.]$$pNanomaterials (Basel)$$tNanomaterials$$x2079-4991
000136279 8564_ $$s9467195$$uhttps://zaguan.unizar.es/record/136279/files/texto_completo.pdf$$yVersión publicada
000136279 8564_ $$s2709510$$uhttps://zaguan.unizar.es/record/136279/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000136279 909CO $$ooai:zaguan.unizar.es:136279$$particulos$$pdriver
000136279 951__ $$a2025-09-22-14:38:42
000136279 980__ $$aARTICLE