000136292 001__ 136292
000136292 005__ 20260112133250.0
000136292 0247_ $$2doi$$a10.1016/j.isci.2024.110435
000136292 0248_ $$2sideral$$a139184
000136292 037__ $$aART-2024-139184
000136292 041__ $$aeng
000136292 100__ $$aCasado-Combreras, Miguel Á.
000136292 245__ $$aCytochrome c prompts the recruitment of its nuclear partners SET/TAF-Iß and NPM1 into biomolecular condensates
000136292 260__ $$c2024
000136292 5060_ $$aAccess copy available to the general public$$fUnrestricted
000136292 5203_ $$aCompartmentalization of proteins by liquid-liquid phase separation (LLPS) is used by cells to control biochemical reactions spatially and temporally. Among them, the recruitment of proteins to DNA foci and nucleolar trafficking occur by biomolecular condensation. Within this frame, the oncoprotein SET/TAF-Iβ plays a key role in both chromatin remodeling and DNA damage response, as does nucleophosmin (NPM1) which indeed participates in nucleolar ribosome synthesis. Whereas phase separation by NPM1 is widely characterized, little is known about that undergone by SET/TAF-Iβ. Here, we show that SET/TAF-Iβ experiences phase separation together with respiratory cytochrome c (Cc), which translocates to the nucleus upon DNA damage. Here we report the molecular mechanisms governing Cc-induced phase separation of SET/TAF-Iβ and NPM1, where two lysine-rich clusters of Cc are essential to recognize molecular surfaces on both partners in a specific and coordinated manner. Cc thus emerges as a small, globular protein with sequence-encoded heterotypic phase-separation properties.
000136292 536__ $$9info:eu-repo/grantAgreement/ES/MCIU/FPU18-06577$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-126663NB-I00
000136292 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000136292 590__ $$a4.1$$b2024
000136292 592__ $$a1.363$$b2024
000136292 591__ $$aMULTIDISCIPLINARY SCIENCES$$b22 / 135 = 0.163$$c2024$$dQ1$$eT1
000136292 593__ $$aMultidisciplinary$$c2024$$dQ1
000136292 594__ $$a6.9$$b2024
000136292 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000136292 700__ $$0(orcid)0000-0001-5702-4538$$aVelázquez-Campoy, Adrián$$uUniversidad de Zaragoza
000136292 700__ $$aMartinho, Marlène
000136292 700__ $$aBelle, Valérie
000136292 700__ $$aDe la Rosa, Miguel A.
000136292 700__ $$aDíaz-Moreno, Irene
000136292 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000136292 773__ $$g27, 8 (2024), 110435$$piScience$$tISCIENCE$$x2589-0042
000136292 8564_ $$s7423041$$uhttps://zaguan.unizar.es/record/136292/files/texto_completo.pdf$$yVersión publicada
000136292 8564_ $$s1600625$$uhttps://zaguan.unizar.es/record/136292/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000136292 909CO $$ooai:zaguan.unizar.es:136292$$particulos$$pdriver
000136292 951__ $$a2026-01-12-12:53:58
000136292 980__ $$aARTICLE