000144625 001__ 144625
000144625 005__ 20250923084421.0
000144625 0247_ $$2doi$$a10.1021/acs.biomac.4c00551
000144625 0248_ $$2sideral$$a139434
000144625 037__ $$aART-2024-139434
000144625 041__ $$aeng
000144625 100__ $$aHernández-Hatibi, Soraya$$uUniversidad de Zaragoza
000144625 245__ $$aPolydopamine interfacial coating for stable Tumor-on-a-Chip models: application for Pancreatic Ductal Adenocarcinoma
000144625 260__ $$c2024
000144625 5060_ $$aAccess copy available to the general public$$fUnrestricted
000144625 5203_ $$aAddressing current challenges in solid tumor research requires advanced in vitro three-dimensional (3D) cellular models that replicate the inherently 3D architecture and microenvironment of tumor tissue, including the extracellular matrix (ECM). However, tumor cells exert mechanical forces that can disrupt the physical integrity of the matrix in long-term 3D culture. Therefore, it is necessary to find the optimal balance between cellular forces and the preservation of matrix integrity. This work proposes using polydopamine (PDA) coating for 3D microfluidic cultures of pancreatic cancer cells to overcome matrix adhesion challenges to sustain representative tumor 3D cultures. Using PDA’s distinctive adhesion and biocompatibility, our model uses type I collagen hydrogels seeded with different pancreatic cancer cell lines, prompting distinct levels of matrix deformation and contraction. Optimizing the PDA coating enhances the adhesion and stability of collagen hydrogels within microfluidic devices, achieving a balance between the disruptive forces of tumor cells on matrix integrity and the maintenance of long-term 3D cultures. The findings reveal how this tension appears to be a critical determinant in spheroid morphology and growth dynamics. Stable and prolonged 3D culture platforms are crucial for understanding solid tumor cell behavior, dynamics, and responses within a controlled microenvironment. This advancement ultimately offers a powerful tool for drug screening, personalized medicine, and wider cancer therapeutics strategies.
000144625 536__ $$9info:eu-repo/grantAgreement/EC/H2020/101018587/EU/Individual and Collective Migration of the Immune Cellular System/ICoMICS$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101018587-ICoMICS$$9info:eu-repo/grantAgreement/ES/MICINN-AEI-FEDER/PID2021-122409OB-C21$$9info:eu-repo/grantAgreement/ES/MICINN/PRE2019-090264$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2021-033490-I
000144625 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000144625 590__ $$a5.4$$b2024
000144625 592__ $$a1.142$$b2024
000144625 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b61 / 319 = 0.191$$c2024$$dQ1$$eT1
000144625 593__ $$aBioengineering$$c2024$$dQ1
000144625 591__ $$aPOLYMER SCIENCE$$b13 / 94 = 0.138$$c2024$$dQ1$$eT1
000144625 593__ $$aPolymers and Plastics$$c2024$$dQ1
000144625 591__ $$aCHEMISTRY, ORGANIC$$b3 / 57 = 0.053$$c2024$$dQ1$$eT1
000144625 593__ $$aMaterials Chemistry$$c2024$$dQ1
000144625 593__ $$aBiomaterials$$c2024$$dQ1
000144625 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000144625 700__ $$aGuerrero, Pedro Enrique
000144625 700__ $$0(orcid)0000-0002-9864-7683$$aGarcía-Aznar, José Manuel$$uUniversidad de Zaragoza
000144625 700__ $$0(orcid)0000-0001-7062-9099$$aGarcía-Gareta, Elena$$uUniversidad de Zaragoza
000144625 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000144625 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000144625 7102_ $$15001$$2065$$aUniversidad de Zaragoza$$bDpto. Ciencia Tecnol.Mater.Fl.$$cÁrea Cienc.Mater. Ingen.Metal.
000144625 773__ $$g25, 8 (2024), 5169-5180$$pBiomacromolecules$$tBIOMACROMOLECULES$$x1525-7797
000144625 8564_ $$s9342779$$uhttps://zaguan.unizar.es/record/144625/files/texto_completo.pdf$$yVersión publicada
000144625 8564_ $$s3340706$$uhttps://zaguan.unizar.es/record/144625/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000144625 909CO $$ooai:zaguan.unizar.es:144625$$particulos$$pdriver
000144625 951__ $$a2025-09-22-14:36:03
000144625 980__ $$aARTICLE