000144630 001__ 144630 000144630 005__ 20240829125213.0 000144630 0247_ $$2doi$$a10.1016/j.microc.2024.111296 000144630 0248_ $$2sideral$$a139463 000144630 037__ $$aART-2024-139463 000144630 041__ $$aeng 000144630 100__ $$0(orcid)0000-0002-9181-2105$$aGiménez-Ingalaturre, Ana C. 000144630 245__ $$aBactericidal activity of silver nanoparticles: An analytical approach based on single cell and single particle inductively coupled plasma mass spectrometry analysis to determine silver species involved 000144630 260__ $$c2024 000144630 5060_ $$aAccess copy available to the general public$$fUnrestricted 000144630 5203_ $$aThe bactericidal activity of silver nanoparticles is supported by a large number of studies, but their action mechanisms are still a controversial issue due to the role of the silver (I) released from the nanoparticles. In this study, direct analytical methods for detection and identification of dissolved and nanoparticulate silver based on single cell and single particle inductively coupled plasma mass spectrometry (ICP-MS) and hydrodynamic chromatography ICP-MS, in combination with alkaline digestions, have been used. Detection of silver species in Escherichia coli bacteria exposed to silver allowed to confirm the different bactericidal activity associated with silver nanoparticles of different sizes. In the case of 10 nm silver nanoparticles, a combined ion-particle action would be responsible for bactericidal effect, since ionic silver was not detected in the culture medium and both dissolved and particulate silver were detected in the exposed bacteria. On the other hand, bacteria did not internalize 60 nm silver nanoparticles and their bactericidal activity was related to the ionic silver released in the culture medium. 000144630 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B43-23R$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E29-23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-123203OB-I00$$9info:eu-repo/grantAgreement/ES/NextGenerationEU/MZ-240621 000144630 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/ 000144630 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000144630 700__ $$0(orcid)0000-0002-7931-3901$$aAbad-Álvaro, Isabel$$uUniversidad de Zaragoza 000144630 700__ $$0(orcid)0000-0002-8066-7117$$aBakir, Mariam 000144630 700__ $$0(orcid)0000-0002-9981-9045$$aChueca, Patricia$$uUniversidad de Zaragoza 000144630 700__ $$0(orcid)0000-0003-0765-7227$$aGoñi, Pilar$$uUniversidad de Zaragoza 000144630 700__ $$0(orcid)0000-0002-4169-0357$$aLaborda, Francisco$$uUniversidad de Zaragoza 000144630 7102_ $$11011$$2660$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Parasitología 000144630 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica 000144630 7102_ $$11001$$2X$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Técnica. Lab. y Talleres 000144630 773__ $$g205 (2024), 111296 [10 pp.]$$pMicrochem. j.$$tMICROCHEMICAL JOURNAL$$x0026-265X 000144630 8564_ $$s3067927$$uhttps://zaguan.unizar.es/record/144630/files/texto_completo.pdf$$yVersión publicada 000144630 8564_ $$s2408567$$uhttps://zaguan.unizar.es/record/144630/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000144630 909CO $$ooai:zaguan.unizar.es:144630$$particulos$$pdriver 000144630 951__ $$a2024-08-29-10:46:07 000144630 980__ $$aARTICLE