000144731 001__ 144731
000144731 005__ 20240906111328.0
000144731 0247_ $$2doi$$a10.3389/fimmu.2024.1386590
000144731 0248_ $$2sideral$$a139486
000144731 037__ $$aART-2024-139486
000144731 041__ $$aeng
000144731 100__ $$aGuisasola-Serrano, Aitor
000144731 245__ $$aIdentifying transcriptomic profiles in ovine spleen after repetitive vaccination
000144731 260__ $$c2024
000144731 5060_ $$aAccess copy available to the general public$$fUnrestricted
000144731 5203_ $$aAluminum hydroxide has long been employed as a vaccine adjuvant for its safety profile, although its precise mechanism of action remains elusive. In this study, we investigated the transcriptomic responses in sheep spleen following repetitive vaccination with aluminum adjuvanted vaccines and aluminum hydroxide alone. Notably, this work represents the first exploration of the sheep spleen transcriptome in such conditions. Animals were splitted in 3 treatment groups: vaccine group, adjuvant alone group and control group. A total of 18 high-depth RNA-seq libraries were sequenced, resulting in a rich dataset which also allowed isoform-level analysis. The comparisons between vaccine-treated and control groups (V vs C) as well as between vaccine-treated and adjuvant-alone groups (V vs A) revealed significant alterations in gene expression profiles, including protein coding genes and long non-coding RNAs. Among the differentially expressed genes, many were associated with processes such as endoplasmic reticulum (ER) stress, immune response and cell cycle. The analysis of co-expression modules further indicated a correlation between vaccine treatment and genes related to ER stress and unfolded protein response. Surprisingly, adjuvant-alone treatment had little impact on the spleen transcriptome. Additionally, the role of alternative splicing in the immune response was explored. We identified isoform switches in genes associated with immune regulation and inflammation, potentially influencing protein function. In conclusion, this study provides valuable insights into the transcriptomic changes in sheep spleen following vaccination with aluminum adjuvanted vaccines and aluminum hydroxide alone. These findings shed light on the molecular mechanisms underlying vaccine-induced immune responses and emphasize the significance of antigenic components in aluminum adjuvant mechanism of action. Furthermore, the analysis of alternative splicing revealed an additional layer of complexity in the immune response to vaccination in a livestock species.
000144731 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2013-49137-C3-3-R
000144731 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000144731 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000144731 700__ $$aBilbao-Arribas, Martín
000144731 700__ $$aVarela-Martínez, Endika
000144731 700__ $$aAbendaño, Naiara
000144731 700__ $$0(orcid)0000-0002-8133-2124$$aPérez, Marta$$uUniversidad de Zaragoza
000144731 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, Lluís$$uUniversidad de Zaragoza
000144731 700__ $$aJugo, Begoña Marina
000144731 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000144731 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000144731 773__ $$g15 (2024), 1386590 [13 pp.]$$pFront. immunol.$$tFrontiers in Immunology$$x1664-3224
000144731 8564_ $$s4182567$$uhttps://zaguan.unizar.es/record/144731/files/texto_completo.pdf$$yVersión publicada
000144731 8564_ $$s2058177$$uhttps://zaguan.unizar.es/record/144731/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000144731 909CO $$ooai:zaguan.unizar.es:144731$$particulos$$pdriver
000144731 951__ $$a2024-09-06-10:24:57
000144731 980__ $$aARTICLE