000144864 001__ 144864
000144864 005__ 20240912134241.0
000144864 0247_ $$2doi$$a10.3390/antiox13080908
000144864 0248_ $$2sideral$$a139719
000144864 037__ $$aART-2024-139719
000144864 041__ $$aeng
000144864 100__ $$0(orcid)0009-0001-5345-6947$$aQuero, Javier$$uUniversidad de Zaragoza
000144864 245__ $$aBiological Properties of Boletus edulis Extract on Caco-2 Cells: Antioxidant, Anticancer, and Anti-Inflammatory Effects
000144864 260__ $$c2024
000144864 5060_ $$aAccess copy available to the general public$$fUnrestricted
000144864 5203_ $$aBoletus edulis (BE) is a mushroom well known for its taste, nutritional value, and medicinal properties. The objective of this work was to study the biological effects of BE extracts on human colon carcinoma cells (Caco-2), evaluating parameters related to oxidative stress and inflammation. In this study, a hydroethanolic extract of BE was obtained by ohmic heating green technology. The obtained BE extracts are mainly composed of sugars (mainly trehalose), phenolic compounds (taxifolin, rutin, and ellagic acid), and minerals (K, P, Mg, Na, Ca, Zn, Se, etc.). The results showed that BE extracts were able to reduce cancer cell proliferation by the induction of cell cycle arrest at the G0/G1 stage, as well as cell death by autophagy and apoptosis, the alteration of mitochondrial membrane potential, and caspase-3 activation. The extracts modified the redox balance of the cell by increasing the ROS levels associated with a decrease in the thioredoxin reductase activity. Similarly, BE extracts attenuated Caco-2 inflammation by reducing both iNOS and COX-2 mRNA expression and COX-2 protein expression. In addition, BE extracts protected the intestine from the oxidative stress induced by H2O2. Therefore, this study provides information on the potential use of BE bioactive compounds as anticancer therapeutic agents and as functional ingredients to prevent oxidative stress in the intestinal barrier.
000144864 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B16-23R$$9info:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/MCINN/PID2022-136414OB-I00$$9info:eu-repo/grantAgreement/EUR/SUDOE/INTERREG/NEWPOWER-S1-1.1-E01116
000144864 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000144864 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000144864 700__ $$aPaesa, Mónica$$uUniversidad de Zaragoza
000144864 700__ $$aMorales, Carmen
000144864 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia
000144864 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, Jesús$$uUniversidad de Zaragoza
000144864 700__ $$aTeixeira, José António
000144864 700__ $$aFerreira-Santos, Pedro
000144864 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, María Jesús$$uUniversidad de Zaragoza
000144864 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000144864 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000144864 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000144864 773__ $$g13, 8 (2024), 908 [22 pp.]$$pAntioxidants$$tAntioxidants$$x2076-3921
000144864 8564_ $$s1831351$$uhttps://zaguan.unizar.es/record/144864/files/texto_completo.pdf$$yVersión publicada
000144864 8564_ $$s2635740$$uhttps://zaguan.unizar.es/record/144864/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000144864 909CO $$ooai:zaguan.unizar.es:144864$$particulos$$pdriver
000144864 951__ $$a2024-09-12-13:06:02
000144864 980__ $$aARTICLE