144864 20260217205553.0 doi 10.3390/antiox13080908 sideral 139719 ART-2024-139719 eng Quero, Javier Universidad de Zaragoza (orcid)0009-0001-5345-6947 Biological Properties of Boletus edulis Extract on Caco-2 Cells: Antioxidant, Anticancer, and Anti-Inflammatory Effects 2024 Access copy available to the general public Unrestricted Boletus edulis (BE) is a mushroom well known for its taste, nutritional value, and medicinal properties. The objective of this work was to study the biological effects of BE extracts on human colon carcinoma cells (Caco-2), evaluating parameters related to oxidative stress and inflammation. In this study, a hydroethanolic extract of BE was obtained by ohmic heating green technology. The obtained BE extracts are mainly composed of sugars (mainly trehalose), phenolic compounds (taxifolin, rutin, and ellagic acid), and minerals (K, P, Mg, Na, Ca, Zn, Se, etc.). The results showed that BE extracts were able to reduce cancer cell proliferation by the induction of cell cycle arrest at the G0/G1 stage, as well as cell death by autophagy and apoptosis, the alteration of mitochondrial membrane potential, and caspase-3 activation. The extracts modified the redox balance of the cell by increasing the ROS levels associated with a decrease in the thioredoxin reductase activity. Similarly, BE extracts attenuated Caco-2 inflammation by reducing both iNOS and COX-2 mRNA expression and COX-2 protein expression. In addition, BE extracts protected the intestine from the oxidative stress induced by H2O2. Therefore, this study provides information on the potential use of BE bioactive compounds as anticancer therapeutic agents and as functional ingredients to prevent oxidative stress in the intestinal barrier. info:eu-repo/grantAgreement/ES/DGA/B16-23R info:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012 info:eu-repo/grantAgreement/ES/MCINN/PID2022-136414OB-I00 info:eu-repo/grantAgreement/EUR/SUDOE/INTERREG/NEWPOWER-S1-1.1-E01116 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 6.6 2024 BIOCHEMISTRY & MOLECULAR BIOLOGY 46 / 320 = 0.144 2024 Q1 T1 FOOD SCIENCE & TECHNOLOGY 22 / 182 = 0.121 2024 Q1 T1 CHEMISTRY, MEDICINAL 8 / 72 = 0.111 2024 Q1 T1 1.484 2024 Biochemistry 2024 Q1 Clinical Biochemistry 2024 Q1 Physiology 2024 Q1 Food Science 2024 Q1 Molecular Biology 2024 Q1 Cell Biology 2024 Q2 12.4 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Paesa, Mónica Universidad de Zaragoza Morales, Carmen Mendoza, Gracia (orcid)0000-0003-2293-363X Osada, Jesús Universidad de Zaragoza (orcid)0000-0002-8251-8457 Teixeira, José António Ferreira-Santos, Pedro Rodríguez-Yoldi, María Jesús Universidad de Zaragoza (orcid)0000-0002-3595-7668 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 13, 8 (2024), 908 [22 pp.] Antioxidants Antioxidants 2076-3921 1831351 http://zaguan.unizar.es/record/144864/files/texto_completo.pdf Versión publicada 2635740 http://zaguan.unizar.es/record/144864/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:144864 articulos driver 2026-02-17-20:41:20 ARTICLE