000144969 001__ 144969
000144969 005__ 20241220120718.0
000144969 0247_ $$2doi$$a10.1007/s00216-024-05401-x
000144969 0248_ $$2sideral$$a139768
000144969 037__ $$aART-2024-139768
000144969 041__ $$aeng
000144969 100__ $$aDomínguez, M.$$uUniversidad de Zaragoza
000144969 245__ $$aColorimetric enzymatic rapid test for the determination of atropine in baby food using a smartphone
000144969 260__ $$c2024
000144969 5060_ $$aAccess copy available to the general public$$fUnrestricted
000144969 5203_ $$aA method for the enzymatic determination of atropine has been developed, which is based on a sequence of reactions involving (1) the hydrolysis of atropine to give tropine; (2) the enzymatic oxidation of tropine with NAD (catalysed by tropinone reductase); and (3) an indicator reaction, in which the NADH previously formed reduces the dye iodonitrotetrazolium chloride (INT) to a reddish species, the reaction catalysed by diaphorase. The method was first developed in solution (linear response range from 2.4 × 10−6 M to 1.0 × 10−4 M). It was then implemented in cellulose platforms to develop a rapid test where the determination is made by measuring the RGB coordinates of the platforms using a smartphone-based device. The device is based on the integrating sphere concept and contains a light source to avoid external illumination effects. The smartphone is controlled by an app that allows a calibration line to be generated and the atropine concentration to be quantified; moreover, since the app normalizes the CCD response of the smartphone, the results and calibrations obtained with different smartphones are similar and can be shared. Using the G coordinate, the results were shown to have a linear response with the concentration of atropine ranging from 1.2 × 10−5 M to 3.0 × 10−4 M with an RSD of 1.4% (n = 5). The method has been applied to the determination of atropine in baby food and buckwheat samples with good results.
000144969 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E25-23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-139235OB-I00
000144969 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000144969 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000144969 700__ $$aMoraru, D.
000144969 700__ $$aLasso, S.
000144969 700__ $$0(orcid)0000-0002-3906-4576$$aSanz-Vicente, I.$$uUniversidad de Zaragoza
000144969 700__ $$0(orcid)0000-0002-7902-6005$$aMarcos, S. de$$uUniversidad de Zaragoza
000144969 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, J.$$uUniversidad de Zaragoza
000144969 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000144969 773__ $$g416 (2024), 7317–7323$$pAnal. Bioanal. Chem.$$tAnalytical and Bioanalytical Chemistry$$x1618-2642
000144969 8564_ $$s998473$$uhttps://zaguan.unizar.es/record/144969/files/texto_completo.pdf$$yVersión publicada
000144969 8564_ $$s2518524$$uhttps://zaguan.unizar.es/record/144969/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000144969 909CO $$ooai:zaguan.unizar.es:144969$$particulos$$pdriver
000144969 951__ $$a2024-12-20-12:05:36
000144969 980__ $$aARTICLE