144969 20260217205555.0 doi 10.1007/s00216-024-05401-x sideral 139768 ART-2024-139768 eng Domínguez, M. Universidad de Zaragoza Colorimetric enzymatic rapid test for the determination of atropine in baby food using a smartphone 2024 Access copy available to the general public Unrestricted A method for the enzymatic determination of atropine has been developed, which is based on a sequence of reactions involving (1) the hydrolysis of atropine to give tropine; (2) the enzymatic oxidation of tropine with NAD (catalysed by tropinone reductase); and (3) an indicator reaction, in which the NADH previously formed reduces the dye iodonitrotetrazolium chloride (INT) to a reddish species, the reaction catalysed by diaphorase. The method was first developed in solution (linear response range from 2.4 × 10−6 M to 1.0 × 10−4 M). It was then implemented in cellulose platforms to develop a rapid test where the determination is made by measuring the RGB coordinates of the platforms using a smartphone-based device. The device is based on the integrating sphere concept and contains a light source to avoid external illumination effects. The smartphone is controlled by an app that allows a calibration line to be generated and the atropine concentration to be quantified; moreover, since the app normalizes the CCD response of the smartphone, the results and calibrations obtained with different smartphones are similar and can be shared. Using the G coordinate, the results were shown to have a linear response with the concentration of atropine ranging from 1.2 × 10−5 M to 3.0 × 10−4 M with an RSD of 1.4% (n = 5). The method has been applied to the determination of atropine in baby food and buckwheat samples with good results. info:eu-repo/grantAgreement/ES/DGA/E25-23R info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2022-139235OB-I00 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 3.8 2024 BIOCHEMICAL RESEARCH METHODS 21 / 86 = 0.244 2024 Q1 T1 CHEMISTRY, ANALYTICAL 31 / 111 = 0.279 2024 Q2 T1 0.716 2024 Biochemistry 2024 Q2 Analytical Chemistry 2024 Q2 7.9 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Moraru, D. Lasso, S. Sanz-Vicente, I. Universidad de Zaragoza (orcid)0000-0002-3906-4576 Marcos, S. de Universidad de Zaragoza (orcid)0000-0002-7902-6005 Galbán, J. Universidad de Zaragoza (orcid)0000-0002-8973-5104 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 416 (2024), 7317–7323 Anal. Bioanal. Chem. Analytical and Bioanalytical Chemistry 1618-2642 998473 http://zaguan.unizar.es/record/144969/files/texto_completo.pdf Versión publicada 2518524 http://zaguan.unizar.es/record/144969/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:144969 articulos driver 2026-02-17-20:42:03 ARTICLE