000144970 001__ 144970
000144970 005__ 20240920135617.0
000144970 0247_ $$2doi$$a10.1016/j.theriogenology.2024.07.012
000144970 0248_ $$2sideral$$a139802
000144970 037__ $$aART-2024-139802
000144970 041__ $$aeng
000144970 100__ $$aMerchán, M.
000144970 245__ $$aDensitometric analysis of GnRH and IBA1 immunocytochemistry in the basal ventromedial hypothalamus of the ewe
000144970 260__ $$c2024
000144970 5060_ $$aAccess copy available to the general public$$fUnrestricted
000144970 5203_ $$aGonadotropin releasing hormone (GnRH) synthesis and secretion regulates seasonal fertility. In the brain, the distribution of GnRH-positive neurons is diffuse, hindering efforts to monitor variations in its cellular and tissue levels. Here, we aim at assessing GnRH immunoreactivity in nuclei responsible for seasonal fertility regulation (SFR) within the posterior, anterior, and preoptic areas of the basal hypothalamus during estrous in ewes. We detected reaction products in the ventromedial basal hypothalamus in neurons, nerve fibers, non-neuronal immunoreactive bodies, and diffuse interstitial areas. Immunoreactivity correlated with the distribution of the main SFR nuclei in the arcuate, retrochiasmatic, periventricular, medial preoptic, supraoptic, and preoptic areas. By independent component analysis density segmentation and by interferential contrast, we identified GnRH non-neuronal positive bodies as microglial cells encapsulated within a dense halo of reaction products. These GnRH-positive microglial cells were distributed in patches and rows throughout the basal ventromedial hypothalamus, suggesting their role in paracrine or juxtacrine signaling. Moreover, as shown by ionized calcium-binding adaptor molecule 1 (IBA1) immunocytochemistry, the distribution of GnRH reaction products overlapped with the microglial dense reactive zones. Therefore, our findings support the assertion that a combined densitometric analysis of GnRH and IBA1 immunocytochemistry enables activity mapping for monitoring seasonal changes following experimental interventions.
000144970 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-117266RB-C21
000144970 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000144970 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000144970 700__ $$aPlaza, I.
000144970 700__ $$aNieto, J.
000144970 700__ $$aPlaza, J.
000144970 700__ $$0(orcid)0000-0003-2827-3054$$aAbecia, J.A.$$uUniversidad de Zaragoza
000144970 700__ $$aPalacios, C.
000144970 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000144970 773__ $$g227 (2024), 112-119$$p1879-3231]$$tTHERIOGENOLOGY$$x0093-691X
000144970 8564_ $$s15102073$$uhttps://zaguan.unizar.es/record/144970/files/texto_completo.pdf$$yVersión publicada
000144970 8564_ $$s2372876$$uhttps://zaguan.unizar.es/record/144970/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000144970 909CO $$ooai:zaguan.unizar.es:144970$$particulos$$pdriver
000144970 951__ $$a2024-09-20-13:01:37
000144970 980__ $$aARTICLE