000144974 001__ 144974
000144974 005__ 20240920135617.0
000144974 0247_ $$2doi$$a10.3390/biom14070810
000144974 0248_ $$2sideral$$a139814
000144974 037__ $$aART-2024-139814
000144974 041__ $$aeng
000144974 100__ $$aMedel-Martinez, Ana
000144974 245__ $$aIsolation and characterization of milk exosomes for use in advanced therapies
000144974 260__ $$c2024
000144974 5060_ $$aAccess copy available to the general public$$fUnrestricted
000144974 5203_ $$aExosomes are cell-derived extracellular vesicles (EVs) with diameters between 30 and 120 nm. In recent years, several studies have evaluated the therapeutic potential of exosomes derived from different fluids due to their low immunogenicity and high biocompatibility. However, producing exosomes on a large scale is still challenging. One of the fluids from which they could be isolated in large quantities is milk. Moreover, regeneration is a well-known property of milk. The present work seeks to optimize a method for isolating exosomes from bovine and human milk, comparing different storage conditions and different extraction protocols. We found differences in the yield extraction associated with pre-storage milk conditions and observed some differences according to the processing agent. When we removed milk fat globules and added rennet before freezing, we obtained a cleaner final fraction. In summary, we attempted to optimize a rennet-based new milk–exosome isolation method and concluded that pre-treatment, followed by freezing of samples, yielded the best exosome population.
000144974 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII DTS21-00130$$9info:eu-repo/grantAgreement/ES/ISCIII/PI19-01007$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-125948OB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-099343-B-100
000144974 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000144974 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000144974 700__ $$aRedrado-Osta, Ana
000144974 700__ $$aCrespo-Barreda, Alejandra
000144974 700__ $$0(orcid)0000-0001-8762-5457$$aSancho-Albero, Maria$$uUniversidad de Zaragoza
000144974 700__ $$aSánchez, Lourdes
000144974 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, Víctor$$uUniversidad de Zaragoza
000144974 700__ $$aPardo, María
000144974 700__ $$ade la Vieja, Antonio
000144974 700__ $$0(orcid)0000-0003-4160-9720$$aMartín-Duque, Pilar$$uUniversidad de Zaragoza
000144974 7102_ $$11013$$2275$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Estomatología
000144974 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000144974 773__ $$g14, 7 (2024), 810 [17 pp.]$$tBiomolecules$$x2218-273X
000144974 8564_ $$s3797465$$uhttps://zaguan.unizar.es/record/144974/files/texto_completo.pdf$$yVersión publicada
000144974 8564_ $$s2664096$$uhttps://zaguan.unizar.es/record/144974/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000144974 909CO $$ooai:zaguan.unizar.es:144974$$particulos$$pdriver
000144974 951__ $$a2024-09-20-13:01:42
000144974 980__ $$aARTICLE