000144993 001__ 144993
000144993 005__ 20240920135617.0
000144993 0247_ $$2doi$$a10.3390/pathogens13080629
000144993 0248_ $$2sideral$$a139806
000144993 037__ $$aART-2024-139806
000144993 041__ $$aeng
000144993 100__ $$aErnst, Sonja
000144993 245__ $$aCharacterisation of European Field Goat Prion Isolates in Ovine PrP Overexpressing Transgenic Mice (Tgshp IX) Reveals Distinct Prion Strains
000144993 260__ $$c2024
000144993 5060_ $$aAccess copy available to the general public$$fUnrestricted
000144993 5203_ $$aAfter the detection of bovine spongiform encephalopathy (BSE), and a zoonotic transmissible spongiform encephalopathy (TSE) caused by the pathological prion protein (PrPSc) in two goats, the investigation of goat prions became of greater interest. Therefore, a broad collection of European goat TSE isolates, including atypical scrapie, CH1641 and goat BSE as reference prion strains were biochemically characterised and subsequently inoculated into seven rodent models for further analysis (already published results of this comprehensive study are reviewed here for comparative reasons). We report here the histopathological and immunohistochemical data of this goat TSE panel, obtained after the first passage in Tgshp IX (tg-shARQ) mice, which overexpress the ovine prion protein. In addition to the clear-cut discrimination of all reference prion strains from the classical scrapie (CS) isolates, we were further able to determine three categories of CS strains. The investigation further indicates the occurrence of sub-strains that slightly resemble distant TSE strains, such as BSE or CH1641, reinforcing the theory that CS is not a single strain but a mixture of sub-strains, existing at varying extents in one isolate. This study further proved that Tgshp IX is a potent and reliable tool for the in-depth characterisation of prion strains.
000144993 536__ $$9info:eu-repo/grantAgreement/EUR/FP6/FOODCT-2006-36353-Goat-BSE
000144993 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000144993 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000144993 700__ $$aNonno, Romolo
000144993 700__ $$aLangeveld, Jan
000144993 700__ $$aAndreoletti, Olivier
000144993 700__ $$0(orcid)0000-0001-5105-6133$$aAcin, Cristina$$uUniversidad de Zaragoza
000144993 700__ $$aPapasavva-Stylianou, Penelope
000144993 700__ $$aSklaviadis, Theodoros
000144993 700__ $$aAcutis, Pier Luigi
000144993 700__ $$avan Keulen, Lucien
000144993 700__ $$aSpiropoulos, John
000144993 700__ $$aKeller, Markus
000144993 700__ $$aGroschup, Martin H.
000144993 700__ $$aFast, Christine
000144993 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000144993 773__ $$g13, 8 (2024), 629 [23 pp.]$$pPathogens$$tPathogens$$x2076-0817
000144993 8564_ $$s2448507$$uhttps://zaguan.unizar.es/record/144993/files/texto_completo.pdf$$yVersión publicada
000144993 8564_ $$s2735897$$uhttps://zaguan.unizar.es/record/144993/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000144993 909CO $$ooai:zaguan.unizar.es:144993$$particulos$$pdriver
000144993 951__ $$a2024-09-20-13:02:04
000144993 980__ $$aARTICLE