144993 20260217205556.0 doi 10.3390/pathogens13080629 sideral 139806 ART-2024-139806 eng Ernst, Sonja Characterisation of European Field Goat Prion Isolates in Ovine PrP Overexpressing Transgenic Mice (Tgshp IX) Reveals Distinct Prion Strains 2024 Access copy available to the general public Unrestricted After the detection of bovine spongiform encephalopathy (BSE), and a zoonotic transmissible spongiform encephalopathy (TSE) caused by the pathological prion protein (PrPSc) in two goats, the investigation of goat prions became of greater interest. Therefore, a broad collection of European goat TSE isolates, including atypical scrapie, CH1641 and goat BSE as reference prion strains were biochemically characterised and subsequently inoculated into seven rodent models for further analysis (already published results of this comprehensive study are reviewed here for comparative reasons). We report here the histopathological and immunohistochemical data of this goat TSE panel, obtained after the first passage in Tgshp IX (tg-shARQ) mice, which overexpress the ovine prion protein. In addition to the clear-cut discrimination of all reference prion strains from the classical scrapie (CS) isolates, we were further able to determine three categories of CS strains. The investigation further indicates the occurrence of sub-strains that slightly resemble distant TSE strains, such as BSE or CH1641, reinforcing the theory that CS is not a single strain but a mixture of sub-strains, existing at varying extents in one isolate. This study further proved that Tgshp IX is a potent and reliable tool for the in-depth characterisation of prion strains. info:eu-repo/grantAgreement/EUR/FP6/FOODCT-2006-36353-Goat-BSE info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 3.3 2024 MICROBIOLOGY 74 / 163 = 0.454 2024 Q2 T2 0.949 2024 Immunology and Microbiology (miscellaneous) 2024 Q1 Molecular Biology 2024 Q2 Microbiology (medical) 2024 Q2 Immunology and Allergy 2024 Q2 Infectious Diseases 2024 Q2 6.8 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Nonno, Romolo Langeveld, Jan Andreoletti, Olivier Acin, Cristina Universidad de Zaragoza (orcid)0000-0001-5105-6133 Papasavva-Stylianou, Penelope Sklaviadis, Theodoros Acutis, Pier Luigi van Keulen, Lucien Spiropoulos, John Keller, Markus Groschup, Martin H. Fast, Christine 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 13, 8 (2024), 629 [23 pp.] Pathogens Pathogens 2076-0817 2448507 http://zaguan.unizar.es/record/144993/files/texto_completo.pdf Versión publicada 2735897 http://zaguan.unizar.es/record/144993/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:144993 articulos driver 2026-02-17-20:42:31 ARTICLE