000145014 001__ 145014
000145014 005__ 20250923084440.0
000145014 0247_ $$2doi$$a10.1016/j.virol.2024.110223
000145014 0248_ $$2sideral$$a139752
000145014 037__ $$aART-2024-139752
000145014 041__ $$aeng
000145014 100__ $$0(orcid)0000-0002-2497-9028$$aBenito, Alfredo A.
000145014 245__ $$aDetection and subtyping of influenza A virus in porcine clinical samples from Spain in 2020
000145014 260__ $$c2024
000145014 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145014 5203_ $$aA total of 1019 samples collected on 726 Spanish swine farms suffering from outbreaks of respiratory disease were screened for influenza A viruses (IAVs) using a RT-qPCR method. A subset of positive samples was further analyzed using a subtype-specific RT-qPCR method (n: 142) and Sanger sequencing (n: 64). A total of 19.4% samples from 23% farms tested positive, with infection being most common in suckling (53.6%) and weaning pigs (30.2%). Viruses belonging to four HA subtypes (H1av, H1hu, H1pdm, H3) were detected, with subtypes H1avN2, H1huN2 and H1avN1 accounting for over half of the specimens. An optimized protocol with newly designed primers allowed the detection of H3 viruses in a significant number of samples (21%). A comparison of antigenic positions revealed that circulating strains exhibited mutations with vaccine strains in a significant percentage of amino acid residues, both in the NA protein (27.8–43.3%) and particularly in the HA protein (51–75.3%).
000145014 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000145014 590__ $$a2.4$$b2024
000145014 592__ $$a0.741$$b2024
000145014 591__ $$aVIROLOGY$$b23 / 42 = 0.548$$c2024$$dQ3$$eT2
000145014 593__ $$aVirology$$c2024$$dQ2
000145014 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000145014 700__ $$0(orcid)0000-0003-0210-2919$$aMonteagudo, Luis V.$$uUniversidad de Zaragoza
000145014 700__ $$aLázaro-Gaspar, Sofía
000145014 700__ $$aMazas-Cabetas, Luna
000145014 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, Joaquín$$uUniversidad de Zaragoza
000145014 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000145014 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000145014 773__ $$g600 (2024), 110223 [8 pp.]$$pVirology$$tVIROLOGY$$x0042-6822
000145014 8564_ $$s1874043$$uhttps://zaguan.unizar.es/record/145014/files/texto_completo.pdf$$yVersión publicada
000145014 8564_ $$s2540088$$uhttps://zaguan.unizar.es/record/145014/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000145014 909CO $$ooai:zaguan.unizar.es:145014$$particulos$$pdriver
000145014 951__ $$a2025-09-22-14:50:05
000145014 980__ $$aARTICLE