000145070 001__ 145070
000145070 005__ 20240926122722.0
000145070 0247_ $$2doi$$a10.1039/d4nr01955e
000145070 0248_ $$2sideral$$a139865
000145070 037__ $$aART-2024-139865
000145070 041__ $$aeng
000145070 100__ $$0(orcid)0000-0002-3373-9341$$aIdiago-López, Javier
000145070 245__ $$aMembrane-localized magnetic hyperthermia promotes intracellular delivery of cell-impermeant probes
000145070 260__ $$c2024
000145070 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145070 5203_ $$aIn this work, we report the disruptive use of membrane-localized magnetic hyperthermia to promote the internalization of cell-impermeant probes. Under an alternating magnetic field, magnetic nanoparticles (MNPs) immobilized on the cell membrane via bioorthogonal click chemistry act as nanoheaters and lead to the thermal disruption of the plasma membrane, which can be used for internalization of different types of molecules, such as small fluorescent probes and nucleic acids. Noteworthily, no cell death, oxidative stress and alterations of the cell cycle are detected after the thermal stimulus, although cells are able to sense and respond to the thermal stimulus through the expression of different types of heat shock proteins (HSPs). Finally, we demonstrate the utility of this approach for the transfection of cells with a small interference RNA (siRNA), revealing a similar efficacy to a standard transfection method based on the use of cationic lipid-based reagents (such as Lipofectamine), but with lower cell toxicity. These results open the possibility of developing new procedures for “opening and closing” cellular membranes with minimal disturbance of cellular integrity. This on-demand modification of cell membrane permeability could allow the direct intracellular delivery of biologically relevant (bio)molecules, drugs and nanomaterials, thus overcoming traditional endocytosis pathways and avoiding endosomal entrapment.
000145070 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E15-23R$$9info:eu-repo/grantAgreement/ES/MCIU-AEI-FEDER/BIO2017-84246-C2-1-R$$9info:eu-repo/grantAgreement/ES/MCIU/CAS18-00233$$9info:eu-repo/grantAgreement/ES/MCIU/CNS2023-144436$$9info:eu-repo/grantAgreement/ES/MCIU/PCIN-2017-060$$9info:eu-repo/grantAgreement/ES/MCIU/PGC2018-096016-B-I00$$9info:eu-repo/grantAgreement/ES/MCIU/RYC-2019-026860-I
000145070 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000145070 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000145070 700__ $$aFerreira, Daniela
000145070 700__ $$0(orcid)0000-0003-0641-3407$$aAsín, Laura$$uUniversidad de Zaragoza
000145070 700__ $$0(orcid)0000-0002-2861-2469$$aMoros, María
000145070 700__ $$0(orcid)0000-0002-2854-2907$$aArmenia, Ilaria
000145070 700__ $$0(orcid)0000-0001-6170-4237$$aGrazú, Valeria
000145070 700__ $$aFernandes, Alexandra R.
000145070 700__ $$0(orcid)0000-0003-1081-8482$$ade la Fuente, Jesús M.
000145070 700__ $$aBaptista, Pedro V.
000145070 700__ $$0(orcid)0000-0001-5559-8757$$aFratila, Raluca M.
000145070 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000145070 773__ $$g16, 32 (2024), 15176-15195$$pNanoscale$$tNanoscale$$x2040-3364
000145070 8564_ $$s5571943$$uhttps://zaguan.unizar.es/record/145070/files/texto_completo.pdf$$yVersión publicada
000145070 8564_ $$s2623217$$uhttps://zaguan.unizar.es/record/145070/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000145070 909CO $$ooai:zaguan.unizar.es:145070$$particulos$$pdriver
000145070 951__ $$a2024-09-26-10:58:51
000145070 980__ $$aARTICLE