000145076 001__ 145076
000145076 005__ 20240926122722.0
000145076 0247_ $$2doi$$a10.1016/j.actbio.2024.08.028
000145076 0248_ $$2sideral$$a139878
000145076 037__ $$aART-2024-139878
000145076 041__ $$aeng
000145076 100__ $$0(orcid)0000-0001-8946-4829$$aLaita, Nicolás$$uUniversidad de Zaragoza
000145076 245__ $$aA comprehensive experimental analysis of the local passive response across the healthy porcine left ventricle
000145076 260__ $$c2024
000145076 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145076 5203_ $$aThis work provides a comprehensive characterization of porcine myocardial tissue, combining true biaxial (TBx), simple triaxial shear (STS) and confined compression (CC) tests to analyze its elastic behavior under cyclic loads. We expanded this study to different zones of the ventricular free wall, providing insights into the local behavior along the longitudinal and radial coordinates. The aging impact was also assessed by comparing two age groups (4 and 8 months). Resulting data showed that the myocardium exhibits a highly nonlinear hyperelastic and incompressible behavior. We observed an anisotropy ratio of 2-2.4 between averaged peak stresses in TBx tests and 1-0.59-0.40 orthotropy ratios for normalised fiber-sheet-normal peak stresses in STS tests. We obtained a highly incompressible response, reaching volumetric pressures of 2-7 MPa for perfused tissue in CC tests, with notable differences when fluid drainage was allowed, suggesting a high permeability. Regional analysis showed reduced stiffness and anisotropy (20-25%) at the apical region compared to the medial, which we attributed to differences in the fiber field dispersion. Compressibility also increased towards the epicardium and apical regions. Regarding age-related variations, 8-month animals showed stiffer response (at least 25% increase), particularly in directions where the mechanical stress is absorbed by collagenous fibers (more than 90%), as supported by a histological analysis. Although compressibility of perfused tissue remained unchanged, permeability significantly reduced in 8-month-old animals. Our findings offer new insights into myocardial properties, emphasizing on local variations, which can help to get a more realistic understanding of cardiac mechanics in this common animal model. Statement of significance: In this work, we conducted a comprehensive analysis of the passive mechanical behavior of porcine myocardial tissue through biaxial, triaxial shear, and confined compression tests. Unlike previous research, we investigated the variation in mechanical response across the left ventricular free wall, conventionally assumed homogeneous, revealing differences in terms of stiffness and compressibility. Additionally, we evaluated age-related effects on mechanical properties by comparing two age groups, observing significant variations in stiffness and permeability. To date, there has been no such in-depth exploration of myocardial elastic response and compressibility considering regional variations along the wall and may contribute to a better understanding of the cardiac tissue’s passive mechanical response.
000145076 536__ $$9nfo:eu-repo/grantAgreement/ES/AEI/PID2022-140219OB-I00$$9info:eu-repo/grantAgreement/EC/H2020/874827/EU/Computational biomechanics and bioengineering 3D printing to develop a personalized regenerative biological ventricular assist device to provide lasting functional support to damaged hearts/BRAV3$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 874827-BRAV3$$9info:eu-repo/grantAgreement/ES/MCIN/PLEC2021-008127$$9info:eu-repo/grantAgreement/ES/UZ/ICTS NANBIOSIS-U13 Unit-CIBER-BBN
000145076 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000145076 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000145076 700__ $$aAparici-Gil, Alejandro
000145076 700__ $$0(orcid)0000-0001-5348-924X$$aOliván-Viguera, Aida
000145076 700__ $$aPérez-Martínez, Alba$$uUniversidad de Zaragoza
000145076 700__ $$0(orcid)0000-0002-8375-0354$$aMartínez, Miguel Ángel$$uUniversidad de Zaragoza
000145076 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré, Manuel$$uUniversidad de Zaragoza
000145076 700__ $$0(orcid)0000-0002-0664-5024$$aPeña, Estefanía$$uUniversidad de Zaragoza
000145076 7102_ $$15008$$2800$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Teoría Señal y Comunicac.
000145076 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000145076 773__ $$g(2024), [17 pp.]$$pActa Biomater.$$tACTA BIOMATERIALIA$$x1742-7061
000145076 8564_ $$s3457805$$uhttps://zaguan.unizar.es/record/145076/files/texto_completo.pdf$$yVersión publicada
000145076 8564_ $$s2419780$$uhttps://zaguan.unizar.es/record/145076/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000145076 909CO $$ooai:zaguan.unizar.es:145076$$particulos$$pdriver
000145076 951__ $$a2024-09-26-10:58:59
000145076 980__ $$aARTICLE