000145120 001__ 145120 000145120 005__ 20240926122722.0 000145120 0247_ $$2doi$$a10.1016/j.vetpar.2024.110300 000145120 0248_ $$2sideral$$a139931 000145120 037__ $$aART-2024-139931 000145120 041__ $$aeng 000145120 100__ $$aGonzález, Mikel Alexander 000145120 245__ $$aMolecular and morphological analysis revealed a new Lipoptena species (Diptera: Hippoboscidae) in southern Spain harbouring Coxiella burnetii and bacterial endosymbionts 000145120 260__ $$c2024 000145120 5060_ $$aAccess copy available to the general public$$fUnrestricted 000145120 5203_ $$aHippoboscid flies (Diptera: Hippoboscidae) are obligate bloodsucking ectoparasites of animals. In Europe, limited research has been conducted on this family until the recent introduction of the deer ked Lipoptena fortisetosa Maa, 1965. A new species of the genus Lipoptena, Lipoptena andaluciensis sp. nov., was found in southern Spain after extensive sampling with carbon-dioxide baited suction traps. A total of 52 females and 32 males were collected at 29 out of 476 sites examined over eight months in 2023. Lipoptena andaluciensis sp. nov. was characterized morphologically and molecularly. The new Lipoptena species can be differentiated from the closely related L. fortisetosa by size, chaetotaxy of the dorsal and ventral thorax, abdominal plates, and genitalia. Based on DNA-barcoding, our specimens showed the highest similarity with Melophagus ovinus (Linnaeus, 1758) (88.4 %) and with L. fortisetosa (86–88 %). Individual screening of Lipoptena specimens (n = 76) for seven important zoonotic pathogens such as bacteria (Anaplasmataceae family: Bartonella spp., Borrelia spp., Coxiella burnetii and Rickettsia spp.) and protozoans (Babesia spp. and Theileria spp.) by conventional PCR and RT-PCR was performed. DNA of C. burnetii was detected in one specimen, while two other specimens harboured Anaplasmataceae (Wolbachia spp., 100 % homology and another endosymbiont probably related to Arsenophonus sp., 95.3 % homology, respectively), all representing the first records of these bacteria in the Lipoptena spp. from Europe. Carbon dioxide traps probed its effectiveness as a reliable passive method for keds surveillance. Our study highlights the existence of a new Lipoptena species, presumably widely distributed in southern Spain. The role of this species in the transmission cycle of pathogens of medical-veterinary relevance needs to be considered in the area. 000145120 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/ 000145120 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000145120 700__ $$0(orcid)0000-0001-8198-8118$$aRuiz-Arrondo, Ignacio$$uUniversidad de Zaragoza 000145120 700__ $$aMagallanes, Sergio 000145120 700__ $$aObona, Jozef 000145120 700__ $$aRuiz-López, María José 000145120 700__ $$aFiguerola, Jordi 000145120 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000145120 773__ $$g332 (2024), 110300 [11 pp.]$$pVet. parasitol.$$tVETERINARY PARASITOLOGY$$x0304-4017 000145120 8564_ $$s15109064$$uhttps://zaguan.unizar.es/record/145120/files/texto_completo.pdf$$yVersión publicada 000145120 8564_ $$s2461874$$uhttps://zaguan.unizar.es/record/145120/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000145120 909CO $$ooai:zaguan.unizar.es:145120$$particulos$$pdriver 000145120 951__ $$a2024-09-26-10:59:53 000145120 980__ $$aARTICLE