145120 20260217205558.0 doi 10.1016/j.vetpar.2024.110300 sideral 139931 ART-2024-139931 eng González, Mikel Alexander Molecular and morphological analysis revealed a new Lipoptena species (Diptera: Hippoboscidae) in southern Spain harbouring Coxiella burnetii and bacterial endosymbionts 2024 Access copy available to the general public Unrestricted Hippoboscid flies (Diptera: Hippoboscidae) are obligate bloodsucking ectoparasites of animals. In Europe, limited research has been conducted on this family until the recent introduction of the deer ked Lipoptena fortisetosa Maa, 1965. A new species of the genus Lipoptena, Lipoptena andaluciensis sp. nov., was found in southern Spain after extensive sampling with carbon-dioxide baited suction traps. A total of 52 females and 32 males were collected at 29 out of 476 sites examined over eight months in 2023. Lipoptena andaluciensis sp. nov. was characterized morphologically and molecularly. The new Lipoptena species can be differentiated from the closely related L. fortisetosa by size, chaetotaxy of the dorsal and ventral thorax, abdominal plates, and genitalia. Based on DNA-barcoding, our specimens showed the highest similarity with Melophagus ovinus (Linnaeus, 1758) (88.4 %) and with L. fortisetosa (86–88 %). Individual screening of Lipoptena specimens (n = 76) for seven important zoonotic pathogens such as bacteria (Anaplasmataceae family: Bartonella spp., Borrelia spp., Coxiella burnetii and Rickettsia spp.) and protozoans (Babesia spp. and Theileria spp.) by conventional PCR and RT-PCR was performed. DNA of C. burnetii was detected in one specimen, while two other specimens harboured Anaplasmataceae (Wolbachia spp., 100 % homology and another endosymbiont probably related to Arsenophonus sp., 95.3 % homology, respectively), all representing the first records of these bacteria in the Lipoptena spp. from Europe. Carbon dioxide traps probed its effectiveness as a reliable passive method for keds surveillance. Our study highlights the existence of a new Lipoptena species, presumably widely distributed in southern Spain. The role of this species in the transmission cycle of pathogens of medical-veterinary relevance needs to be considered in the area. info:eu-repo/semantics/openAccess by-nc https://creativecommons.org/licenses/by-nc/4.0/deed.es 2.2 2024 VETERINARY SCIENCES 36 / 170 = 0.212 2024 Q1 T1 PARASITOLOGY 21 / 47 = 0.447 2024 Q2 T2 0.606 2024 Veterinary (miscellaneous) 2024 Q1 Medicine (miscellaneous) 2024 Q2 Parasitology 2024 Q2 4.2 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Ruiz-Arrondo, Ignacio Universidad de Zaragoza (orcid)0000-0001-8198-8118 Magallanes, Sergio Obona, Jozef Ruiz-López, María José Figuerola, Jordi 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 332 (2024), 110300 [11 pp.] Vet. parasitol. VETERINARY PARASITOLOGY 0304-4017 15109064 http://zaguan.unizar.es/record/145120/files/texto_completo.pdf Versión publicada 2461874 http://zaguan.unizar.es/record/145120/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:145120 articulos driver 2026-02-17-20:43:35 ARTICLE