000145279 001__ 145279
000145279 005__ 20250923084435.0
000145279 0247_ $$2doi$$a10.1007/s00253-024-13288-y
000145279 0248_ $$2sideral$$a140057
000145279 037__ $$aART-2024-140057
000145279 041__ $$aeng
000145279 100__ $$aSabuquillo, Pilar
000145279 245__ $$aA reliable qPCR technique for detecting viable Xanthomonas arboricola pv. pruni cells
000145279 260__ $$c2024
000145279 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145279 5203_ $$aXanthomonas arboricola pv. pruni (Xap) is the causal agent of bacterial spot of stone fruits and almond (Prunus spp). Detection of Xap is typically carried out using quantitative real-time PCR (qPCR) combined with culture-based isolation. However, qPCR does not differentiate between viable and dead cells, potentially leading to an overestimation of the infective population in a sample. Such overestimation could result in unnecessary phytosanitary measures. The present study aims to develop a specific protocol ideally targeting to detection of only live Xap bacterial cells. To address this challenge, the viable quantitative PCR (v-qPCR) method was evaluated using three nucleic acid-binding dyes: propidium monoazide (PMA), a combination of PMA and ethidium monoazide (EMA), and PMAxx™, an improved version of PMA. PMAxx™ proved to be the most suitable dye for the detection and quantification of living bacterial cells. This methodology was also evaluated in infected plant material over time and can be considered a rapid and reliable alternative to PCR methods for detecting only those putative infective Xap that may pose a risk for Prunus crops.
000145279 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-123600OR-C44
000145279 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000145279 590__ $$a4.3$$b2024
000145279 592__ $$a0.967$$b2024
000145279 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b42 / 177 = 0.237$$c2024$$dQ1$$eT1
000145279 593__ $$aApplied Microbiology and Biotechnology$$c2024$$dQ1
000145279 593__ $$aMedicine (miscellaneous)$$c2024$$dQ1
000145279 593__ $$aBiotechnology$$c2024$$dQ2
000145279 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000145279 700__ $$aBerruete, Isabel M.
000145279 700__ $$aCubero, Jaime
000145279 700__ $$0(orcid)0000-0002-3777-8612$$aPalacio-Bielsa, Ana
000145279 773__ $$g108, 1 (2024), 472 [12 pp.]$$pAppl. microbiol. biotechnol.$$tAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY$$x0175-7598
000145279 8564_ $$s1284440$$uhttps://zaguan.unizar.es/record/145279/files/texto_completo.pdf$$yVersión publicada
000145279 8564_ $$s2322710$$uhttps://zaguan.unizar.es/record/145279/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000145279 909CO $$ooai:zaguan.unizar.es:145279$$particulos$$pdriver
000145279 951__ $$a2025-09-22-14:46:09
000145279 980__ $$aARTICLE