000145380 001__ 145380
000145380 005__ 20241024135331.0
000145380 0247_ $$2doi$$a10.1530/REP-24-0172
000145380 0248_ $$2sideral$$a140260
000145380 037__ $$aART-2024-140260
000145380 041__ $$aeng
000145380 100__ $$aViola, Irene
000145380 245__ $$aExogenous melatonin ameliorates embryo–maternal cross-talk in early pregnancy in sheep
000145380 260__ $$c2024
000145380 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145380 5203_ $$aIn brief
Melatonin plays a crucial role in enhancing reproductive performance in small ruminants. This paper reveals the effects of exogenous melatonin on the placental and endometrial rearrangement in early pregnancy in sheep.
Abstract
Early pregnancy losses cause 25% of pregnancy failures in small ruminants because of asynchrony between conceptus and uterine signals. In this context, melatonin plays a crucial role in sheep reproductive dynamics, but little is known about its effects during the peri-implantation period. We hypothesized that melatonin supports embryo implantation by modulating the uterine microenvironment. This study aimed to assess the effects of exogenous melatonin on the endometrial and early placental rearrangement. Ten multiparous ewes either did (MEL, n = 5) or did not (CTR, n = 5) receive a subcutaneous melatonin implant (18 mg) 50 days before a synchronized mating. On day 21 of pregnancy, the sheep were euthanized. MEL ewes exhibited a higher prolificity rate (2.8 vs 2.0 embryos/ewe) and plasma progesterone levels (3.84 vs 2.96 ng/mL, P < 0.05) than did CTR ewes. Groups did not differ significantly in embryo crown-rump length. MEL placentas had significantly (P < 0.001) more binucleated trophoblast cells in the chorion region, and ovine placental lactogen expression was significantly (P < 0.05) more strongly upregulated than in CTR. Exogenous melatonin increased significantly (P < 0.05) gene expression of angiogenic factors (VEGFA, VEGFR1, IGF1R), IFNAR2, and PR in the caruncular endometrium. Expression of the MT2 receptor in the endometrium and placenta was significantly (P < 0.05) higher in the MEL group. These results indicate that melatonin implants acted differentially on uterine and placental rearrangement. Melatonin increases differentiation in the placenta and induces changes that could promote vessel maturation in the endometrium, suggesting that it enhances the uterine microenvironment in the early stage of pregnancy in sheep.
000145380 536__ $$9info:eu-repo/grantAgreement/ES/DGA/BIOFITER
000145380 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000145380 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000145380 700__ $$0(orcid)0000-0003-2907-0427$$aSosa, Cecilia$$uUniversidad de Zaragoza
000145380 700__ $$aAccornero, Paolo
000145380 700__ $$aManenti, Isabella
000145380 700__ $$aCanto, Francisco
000145380 700__ $$aMiretti, Silvia
000145380 700__ $$0(orcid)0000-0003-2827-3054$$aAbecia, José Alfonso$$uUniversidad de Zaragoza
000145380 700__ $$aToschi, Paola
000145380 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000145380 7102_ $$11002$$2807$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Toxicología
000145380 773__ $$g168, 5 (2024), e240172 [10 pp.]$$pREPRODUCTION$$tREPRODUCTION$$x1470-1626
000145380 8564_ $$s1901802$$uhttps://zaguan.unizar.es/record/145380/files/texto_completo.pdf$$yVersión publicada
000145380 8564_ $$s2375250$$uhttps://zaguan.unizar.es/record/145380/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000145380 909CO $$ooai:zaguan.unizar.es:145380$$particulos$$pdriver
000145380 951__ $$a2024-10-24-12:11:41
000145380 980__ $$aARTICLE