000145473 001__ 145473
000145473 005__ 20241030091919.0
000145473 0247_ $$2doi$$a10.1186/s12870-024-05658-5
000145473 0248_ $$2sideral$$a140319
000145473 037__ $$aART-2024-140319
000145473 041__ $$aeng
000145473 100__ $$aTrunova, Dana
000145473 245__ $$aDoes time matter? Intraspecific diversity of ribosomal RNA genes in lineages of the allopolyploid model grass Brachypodium hybridum with different evolutionary ages
000145473 260__ $$c2024
000145473 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145473 5203_ $$aBackground
Polyploidisation often results in genome rearrangements that may involve changes in both the single-copy sequences and the repetitive genome fraction. In this study, we performed a comprehensive comparative analysis of repetitive DNA, with a particular focus on ribosomal DNA (rDNA), in Brachypodium hybridum (2n = 4x = 30, subgenome composition DDSS), an allotetraploid resulting from a natural cross between two diploid species that resemble the modern B. distachyon (2n = 10; DD) and B. stacei (2n = 20; SS). Taking advantage of the recurrent origin of B. hybridum, we investigated two genotypes, Bhyb26 and ABR113, differing markedly in their evolutionary age (1.4 and 0.14 Mya, respectively) and which resulted from opposite cross directions. To identify the origin of rDNA loci we employed cytogenetic and molecular methods (FISH, gCAPS and Southern hybridisation), phylogenetic and genomic approaches.
Results
Unlike the general maintenance of doubled gene dosage in B. hybridum, the rRNA genes showed a remarkable tendency towards diploidisation at both locus and unit levels. While the partial elimination of 35S rDNA units occurred in the younger ABR113 lineage, unidirectional elimination of the entire locus was observed in the older Bhyb26 lineage. Additionally, a novel 5S rDNA family was amplified in Bhyb26 replacing the parental units. The 35S and 5S rDNA units were preferentially eliminated from the S- and D-subgenome, respectively. Thus, in the more ancient B. hybridum lineage, Bhyb26, 5S and 35S rRNA genes are likely expressed from different subgenomes, highlighting the complexity of polyploid regulatory networks.
Conclusion
Comparative analyses between two B. hybridum lineages of distinct evolutionary ages revealed that although the recent lineage ABR113 exhibited an additive pattern of rDNA loci distribution, the ancient lineage Bhyb26 demonstrated a pronounced tendency toward diploidisation manifested by the reduction in the number of both 35S and 5S loci. In conclusion, the age of the allopolyploid appears to be a decisive factor in rDNA turnover in B. hybridum.
000145473 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-140074NB-I00$$9info:eu-repo/grantAgreement/EUR/MICINN/TED2021-131073B-I00
000145473 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000145473 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000145473 700__ $$aBorowska-Zuchowska, Natalia
000145473 700__ $$aMykhailyk, Serhii
000145473 700__ $$aXia, Kai
000145473 700__ $$aZhu, Yuanbin
000145473 700__ $$aSancho, Ruben
000145473 700__ $$aRojek-Jelonek, Magdalena
000145473 700__ $$aGarcia, Sònia
000145473 700__ $$aWang, Kai
000145473 700__ $$0(orcid)0000-0001-7793-5259$$aCatalan, Pilar$$uUniversidad de Zaragoza
000145473 700__ $$aKovarik, Ales
000145473 700__ $$aHasterok, Robert
000145473 700__ $$aKolano, Bozena
000145473 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica
000145473 773__ $$g24, 1 (2024), 981 [14 p.]$$pBMC PLANT BIOLOGY$$tBMC PLANT BIOLOGY$$x1471-2229
000145473 8564_ $$s5370123$$uhttps://zaguan.unizar.es/record/145473/files/texto_completo.pdf$$yVersión publicada
000145473 8564_ $$s2103843$$uhttps://zaguan.unizar.es/record/145473/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000145473 909CO $$ooai:zaguan.unizar.es:145473$$particulos$$pdriver
000145473 951__ $$a2024-10-30-08:49:10
000145473 980__ $$aARTICLE