000145481 001__ 145481
000145481 005__ 20241030091919.0
000145481 0247_ $$2doi$$a10.3390/nu16183146
000145481 0248_ $$2sideral$$a140335
000145481 037__ $$aART-2024-140335
000145481 041__ $$aeng
000145481 100__ $$aDíez-Sainz, Ester
000145481 245__ $$aPlant miR6262 Modulates the Expression of Metabolic and Thermogenic Genes in Human Hepatocytes and Adipocytes
000145481 260__ $$c2024
000145481 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145481 5203_ $$aBackground: Edible plants have been linked to the mitigation of metabolic disturbances in liver and adipose tissue, including the decrease of lipogenesis and the enhancement of lipolysis and adipocyte browning. In this context, plant microRNAs could be key bioactive molecules underlying the cross-kingdom beneficial effects of plants. This study sought to explore the impact of plant-derived microRNAs on the modulation of adipocyte and hepatocyte genes involved in metabolism and thermogenesis. Methods: Plant miR6262 was selected as a candidate from miRBase for the predicted effect on the regulation of human metabolic genes. Functional validation was conducted after transfection with plant miRNA mimics in HepG2 hepatocytes exposed to free fatty acids to mimic liver steatosis and hMADs cells differentiated into brown-like adipocytes. Results: miR6262 decreases the expression of the predicted target RXRA in the fatty acids-treated hepatocytes and in brown-like adipocytes and affects the expression profile of critical genes involved in metabolism and thermogenesis, including PPARA, G6PC, SREBF1 (hepatocytes) and CIDEA, CPT1M and PLIN1 (adipocytes). Nevertheless, plant miR6262 mimic transfections did not decrease hepatocyte lipid accumulation or stimulate adipocyte browning. Conclusions: these findings suggest that plant miR6262 could have a cross-kingdom regulation relevance through the modulation of human genes involved in lipid and glucose metabolism and thermogenesis in adipocytes and hepatocytes.
000145481 536__ $$9info:eu-repo/grantAgreement/ES/CIBERObn/CB12-03-30002$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/BFU2015-65937-R$$9info:eu-repo/grantAgreement/ES/MINECO/PID2022-141313OB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/PID2022-141766OB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/RTI2018-102205-B-I00
000145481 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000145481 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000145481 700__ $$aMilagro, Fermín I.
000145481 700__ $$aAranaz, Paula
000145481 700__ $$aRiezu-Boj, José I.
000145481 700__ $$0(orcid)0000-0002-1033-6152$$aLorente-Cebrián, Silvia$$uUniversidad de Zaragoza
000145481 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000145481 773__ $$g16, 18 (2024), 3146 [21pp.]$$pNutrients$$tNutrients$$x2072-6643
000145481 8564_ $$s1798049$$uhttps://zaguan.unizar.es/record/145481/files/texto_completo.pdf$$yVersión publicada
000145481 8564_ $$s2715838$$uhttps://zaguan.unizar.es/record/145481/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000145481 909CO $$ooai:zaguan.unizar.es:145481$$particulos$$pdriver
000145481 951__ $$a2024-10-30-08:49:19
000145481 980__ $$aARTICLE