000145590 001__ 145590
000145590 005__ 20241108105822.0
000145590 0247_ $$2doi$$a10.1016/j.rvsc.2024.105423
000145590 0248_ $$2sideral$$a140468
000145590 037__ $$aART-2024-140468
000145590 041__ $$aeng
000145590 100__ $$0(orcid)0000-0002-6806-9990$$aHernaiz, Adelaida$$uUniversidad de Zaragoza
000145590 245__ $$aRNA-sequencing transcriptomic analysis of scrapie-exposed ovine mesenchymal stem cells
000145590 260__ $$c2024
000145590 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145590 5203_ $$aIn neurodegenerative diseases, including prion diseases, cellular models arise as useful tools to study the pathogenic mechanisms occurring in these diseases and to assess the efficacy of potential therapeutic compounds. In the present study, a RNA-sequencing analysis of bone marrow-derived ovine mesenchymal stem cells (oBM-MSCs) exposed to scrapie brain homogenate was performed to try to unravel genes and pathways potentially involved in prion diseases and MSC response mechanisms to prions. The oBM-MSCs were cultured in three different conditions (inoculated with brain homogenate of scrapie-infected sheep, with brain homogenate of healthy sheep and in standard growth conditions without inoculum) that were analysed at two exposure times: 2 and 4 days post-inoculation (dpi). Differentially expressed genes (DEGs) in scrapie-treated oBM-MSCs were found in the two exposure times finding the higher number at 2 dpi, which coincided with the inoculum removal time. Pathways enriched in DEGs were related to biological functions involved in prion toxicity and MSC response to the inflammatory environment of scrapie brain homogenate. Moreover, RNA-sequencing analysis was validated amplifying by RT-qPCR a set of 11 DEGs with functions related with prion propagation and its associated toxicity. Seven of these genes displayed significant expression changes in scrapie-treated cells. These results contribute to the knowledge of the molecular mechanisms behind the early toxicity observed in these cells after prion exposure and to elucidate the response of MSCs to neuroinflammation.
000145590 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A19-20R$$9info:eu-repo/grantAgreement/ES/DGA-FSE/IIU-2023-2017$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00
000145590 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000145590 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000145590 700__ $$0(orcid)0000-0002-1590-3347$$aMarín, Belén$$uUniversidad de Zaragoza
000145590 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, Francisco J.$$uUniversidad de Zaragoza
000145590 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan J.$$uUniversidad de Zaragoza
000145590 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza
000145590 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000145590 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, Inmaculada$$uUniversidad de Zaragoza
000145590 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000145590 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000145590 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000145590 773__ $$g180 (2024), 105423 [11 pp.]$$pRes. Vet. Sci.$$tRESEARCH IN VETERINARY SCIENCE$$x0034-5288
000145590 8564_ $$s4872569$$uhttps://zaguan.unizar.es/record/145590/files/texto_completo.pdf$$yVersión publicada
000145590 8564_ $$s2516993$$uhttps://zaguan.unizar.es/record/145590/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000145590 909CO $$ooai:zaguan.unizar.es:145590$$particulos$$pdriver
000145590 951__ $$a2024-11-08-10:38:34
000145590 980__ $$aARTICLE