145590 20260217205610.0 doi 10.1016/j.rvsc.2024.105423 sideral 140468 ART-2024-140468 eng Hernaiz, Adelaida Universidad de Zaragoza (orcid)0000-0002-6806-9990 RNA-sequencing transcriptomic analysis of scrapie-exposed ovine mesenchymal stem cells 2024 Access copy available to the general public Unrestricted In neurodegenerative diseases, including prion diseases, cellular models arise as useful tools to study the pathogenic mechanisms occurring in these diseases and to assess the efficacy of potential therapeutic compounds. In the present study, a RNA-sequencing analysis of bone marrow-derived ovine mesenchymal stem cells (oBM-MSCs) exposed to scrapie brain homogenate was performed to try to unravel genes and pathways potentially involved in prion diseases and MSC response mechanisms to prions. The oBM-MSCs were cultured in three different conditions (inoculated with brain homogenate of scrapie-infected sheep, with brain homogenate of healthy sheep and in standard growth conditions without inoculum) that were analysed at two exposure times: 2 and 4 days post-inoculation (dpi). Differentially expressed genes (DEGs) in scrapie-treated oBM-MSCs were found in the two exposure times finding the higher number at 2 dpi, which coincided with the inoculum removal time. Pathways enriched in DEGs were related to biological functions involved in prion toxicity and MSC response to the inflammatory environment of scrapie brain homogenate. Moreover, RNA-sequencing analysis was validated amplifying by RT-qPCR a set of 11 DEGs with functions related with prion propagation and its associated toxicity. Seven of these genes displayed significant expression changes in scrapie-treated cells. These results contribute to the knowledge of the molecular mechanisms behind the early toxicity observed in these cells after prion exposure and to elucidate the response of MSCs to neuroinflammation. info:eu-repo/grantAgreement/ES/DGA/A19-20R info:eu-repo/grantAgreement/ES/DGA-FSE/IIU-2023-2017 info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 1.8 2024 VETERINARY SCIENCES 56 / 170 = 0.329 2024 Q2 T1 0.608 2024 Veterinary (miscellaneous) 2024 Q1 4.6 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Marín, Belén Universidad de Zaragoza (orcid)0000-0002-1590-3347 Vázquez, Francisco J. Universidad de Zaragoza (orcid)0000-0002-8712-2275 Badiola, Juan J. Universidad de Zaragoza (orcid)0000-0002-7173-7216 Zaragoza, Pilar Universidad de Zaragoza (orcid)0000-0001-5740-0185 Bolea, Rosa Universidad de Zaragoza (orcid)0000-0002-2746-3932 Martín-Burriel, Inmaculada Universidad de Zaragoza (orcid)0000-0001-6016-4726 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 180 (2024), 105423 [11 pp.] Res. Vet. Sci. RESEARCH IN VETERINARY SCIENCE 0034-5288 4872569 http://zaguan.unizar.es/record/145590/files/texto_completo.pdf Versión publicada 2516993 http://zaguan.unizar.es/record/145590/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:145590 articulos driver 2026-02-17-20:48:39 ARTICLE