000145718 001__ 145718
000145718 005__ 20241122130824.0
000145718 0247_ $$2doi$$a10.3389/fvets.2024.1404770
000145718 0248_ $$2sideral$$a140603
000145718 037__ $$aART-2024-140603
000145718 041__ $$aeng
000145718 100__ $$0(orcid)0000-0001-9773-7460$$aGuijarro, Isabel M.
000145718 245__ $$aIn situ assessment of neuroinflammatory cytokines in different stages of ovine natural prion disease
000145718 260__ $$c2024
000145718 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145718 5203_ $$aIntroduction: According to the neuroinflammatory hypothesis, a cytokine-mediated host innate immune response may be involved in the mechanisms that contribute to the process of neurodegeneration. Specifically, regarding prion diseases, some experimental murine models have evidenced an altered profile of inflammatory intermediaries. However, the local inflammatory response has rarely been assessed, and never in tissues from different natural models throughout the progression of neurodegeneration.
Methods: The aim of this study was to use immunohistochemistry (IHC) to in situ assess the temporal protein expression of several cytokines in the cerebellum of sheep suffering from various clinical stages of scrapie.
Results and discussion: Clear changes in the expression of most of the assessed markers were observed in the affected sheep compared to the healthy control sheep, and from different stages. In summary, this preliminary IHC study focusing in the Purkinje cell layer changes demonstrate that all cytokines or respective receptors studied (IL-1, IL-1R, IL-2R, IL-6, IL-10R, and TNFαR) except for IFNγR are disease-associated signaling proteins showing an increase or decrease in relation to the progression of clinical disease. In the future, this study will be extended to other inflammatory mediators and brain regions, focusing in particular on the release of these inflammatory mediators by astroglial and microglial populations.
000145718 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000145718 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000145718 700__ $$0(orcid)0000-0002-1756-5492$$aGarcés, Moisés$$uUniversidad de Zaragoza
000145718 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan J.$$uUniversidad de Zaragoza
000145718 700__ $$0(orcid)0000-0002-2787-9671$$aMonzón, Marta$$uUniversidad de Zaragoza
000145718 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000145718 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000145718 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000145718 773__ $$g11 (2024), [10 pp.]$$pFront. vet. sci.$$tFrontiers in Veterinary Science$$x2297-1769
000145718 8564_ $$s3673131$$uhttps://zaguan.unizar.es/record/145718/files/texto_completo.pdf$$yVersión publicada
000145718 8564_ $$s2084495$$uhttps://zaguan.unizar.es/record/145718/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000145718 909CO $$ooai:zaguan.unizar.es:145718$$particulos$$pdriver
000145718 951__ $$a2024-11-22-11:53:12
000145718 980__ $$aARTICLE