145718 20260217205603.0 doi 10.3389/fvets.2024.1404770 sideral 140603 ART-2024-140603 eng Guijarro, Isabel M. (orcid)0000-0001-9773-7460 In situ assessment of neuroinflammatory cytokines in different stages of ovine natural prion disease 2024 Access copy available to the general public Unrestricted Introduction: According to the neuroinflammatory hypothesis, a cytokine-mediated host innate immune response may be involved in the mechanisms that contribute to the process of neurodegeneration. Specifically, regarding prion diseases, some experimental murine models have evidenced an altered profile of inflammatory intermediaries. However, the local inflammatory response has rarely been assessed, and never in tissues from different natural models throughout the progression of neurodegeneration. Methods: The aim of this study was to use immunohistochemistry (IHC) to in situ assess the temporal protein expression of several cytokines in the cerebellum of sheep suffering from various clinical stages of scrapie. Results and discussion: Clear changes in the expression of most of the assessed markers were observed in the affected sheep compared to the healthy control sheep, and from different stages. In summary, this preliminary IHC study focusing in the Purkinje cell layer changes demonstrate that all cytokines or respective receptors studied (IL-1, IL-1R, IL-2R, IL-6, IL-10R, and TNFαR) except for IFNγR are disease-associated signaling proteins showing an increase or decrease in relation to the progression of clinical disease. In the future, this study will be extended to other inflammatory mediators and brain regions, focusing in particular on the release of these inflammatory mediators by astroglial and microglial populations. info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 2.9 2024 VETERINARY SCIENCES 19 / 170 = 0.112 2024 Q1 T1 0.783 2024 Veterinary (miscellaneous) 2024 Q1 5.1 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Garcés, Moisés Universidad de Zaragoza (orcid)0000-0002-1756-5492 Badiola, Juan J. Universidad de Zaragoza (orcid)0000-0002-7173-7216 Monzón, Marta Universidad de Zaragoza (orcid)0000-0002-2787-9671 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 11 (2024), [10 pp.] Front. vet. sci. Frontiers in Veterinary Science 2297-1769 3673131 http://zaguan.unizar.es/record/145718/files/texto_completo.pdf Versión publicada 2084495 http://zaguan.unizar.es/record/145718/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:145718 articulos driver 2026-02-17-20:45:52 ARTICLE