| Página principal > Artículos > Molecular Detection of the Grapevine Pathogens Plasmopara viticola and Erysiphe necator from Airborne Inoculum Collector Cyclones > MARC |
000145726 001__ 145726 000145726 005__ 20250923084435.0 000145726 0247_ $$2doi$$a10.3390/agronomy14112619 000145726 0248_ $$2sideral$$a140652 000145726 037__ $$aART-2024-140652 000145726 041__ $$aeng 000145726 100__ $$0(orcid)0000-0001-5136-8639$$aBalduque-Gil, Joaquín 000145726 245__ $$aMolecular Detection of the Grapevine Pathogens Plasmopara viticola and Erysiphe necator from Airborne Inoculum Collector Cyclones 000145726 260__ $$c2024 000145726 5060_ $$aAccess copy available to the general public$$fUnrestricted 000145726 5203_ $$aGrapevine (Vitis vinifera L.) varieties are particularly susceptible to the pathogens downy mildew Plasmopara viticola and powdery mildew Erysiphe necator. Conventional methods for identifying and classifying spores rely on time-consuming microscopic examinations susceptible to human error and requiring qualified personnel. The aim of the present work has focused on the establishment of a protocol for the rapid molecular detection of the fungal species P. viticola and E. necator from adhesive tapes used to trap spores in airborne inoculum collector cyclones. Four DNA extraction methods were tested. Subsequently, molecular detection of both pathogens was performed by validating some of the specific molecular markers available in the literature. PCR with the primers Nad9 cob-F/Nad9 cob-R and Uncin144/Uncin511 showed specific results for P. viticola and E. necator, respectively, and the best results were obtained with the T-CTAB method. The methodology developed in this work could be of great help for relating direct measurement of P. viticola and E. necator airborne inoculum to disease risk and detection of pathogens, which could be integrated into the early diagnosis of these grapevine pathogens, improving existing warning systems such as Decision Support Systems. 000145726 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A08-20R$$9info:eu-repo/grantAgreement/ES/DGA/A11-20R 000145726 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000145726 590__ $$a3.4$$b2024 000145726 592__ $$a0.744$$b2024 000145726 591__ $$aAGRONOMY$$b21 / 129 = 0.163$$c2024$$dQ1$$eT1 000145726 593__ $$aAgronomy and Crop Science$$c2024$$dQ1 000145726 591__ $$aPLANT SCIENCES$$b68 / 273 = 0.249$$c2024$$dQ1$$eT1 000145726 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000145726 700__ $$0(orcid)0000-0002-6727-5928$$aGarcés-Claver, Ana 000145726 700__ $$aPérez-Lamuela, Inés 000145726 700__ $$0(orcid)0000-0003-2980-5454$$aBarriuso-Vargas, Juan J.$$uUniversidad de Zaragoza 000145726 700__ $$aFayos, Oreto 000145726 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal 000145726 773__ $$g14, 11 (2024), 2619 [18 pp.]$$pAgronomy (Basel)$$tAgronomy (Basel)$$x2073-4395 000145726 8564_ $$s7214415$$uhttps://zaguan.unizar.es/record/145726/files/texto_completo.pdf$$yVersión publicada 000145726 8564_ $$s2787398$$uhttps://zaguan.unizar.es/record/145726/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000145726 909CO $$ooai:zaguan.unizar.es:145726$$particulos$$pdriver 000145726 951__ $$a2025-09-22-14:46:25 000145726 980__ $$aARTICLE
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