145726 20260217205605.0 doi 10.3390/agronomy14112619 sideral 140652 ART-2024-140652 eng Balduque-Gil, Joaquín (orcid)0000-0001-5136-8639 Molecular Detection of the Grapevine Pathogens Plasmopara viticola and Erysiphe necator from Airborne Inoculum Collector Cyclones 2024 Access copy available to the general public Unrestricted Grapevine (Vitis vinifera L.) varieties are particularly susceptible to the pathogens downy mildew Plasmopara viticola and powdery mildew Erysiphe necator. Conventional methods for identifying and classifying spores rely on time-consuming microscopic examinations susceptible to human error and requiring qualified personnel. The aim of the present work has focused on the establishment of a protocol for the rapid molecular detection of the fungal species P. viticola and E. necator from adhesive tapes used to trap spores in airborne inoculum collector cyclones. Four DNA extraction methods were tested. Subsequently, molecular detection of both pathogens was performed by validating some of the specific molecular markers available in the literature. PCR with the primers Nad9 cob-F/Nad9 cob-R and Uncin144/Uncin511 showed specific results for P. viticola and E. necator, respectively, and the best results were obtained with the T-CTAB method. The methodology developed in this work could be of great help for relating direct measurement of P. viticola and E. necator airborne inoculum to disease risk and detection of pathogens, which could be integrated into the early diagnosis of these grapevine pathogens, improving existing warning systems such as Decision Support Systems. info:eu-repo/grantAgreement/ES/DGA/A08-20R info:eu-repo/grantAgreement/ES/DGA/A11-20R info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 3.4 2024 AGRONOMY 21 / 129 = 0.163 2024 Q1 T1 PLANT SCIENCES 69 / 273 = 0.253 2024 Q2 T1 0.744 2024 Agronomy and Crop Science 2024 Q1 6.7 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Garcés-Claver, Ana (orcid)0000-0002-6727-5928 Pérez-Lamuela, Inés Barriuso-Vargas, Juan J. Universidad de Zaragoza (orcid)0000-0003-2980-5454 Fayos, Oreto 5011 705 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Área Producción Vegetal 14, 11 (2024), 2619 [18 pp.] Agronomy (Basel) Agronomy (Basel) 2073-4395 7214415 http://zaguan.unizar.es/record/145726/files/texto_completo.pdf Versión publicada 2787398 http://zaguan.unizar.es/record/145726/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:145726 articulos driver 2026-02-17-20:46:23 ARTICLE