Spermiogram, Kinetics, Flow Cytometric Characteristics and DNA Damage Degree in Boar Ejaculates: Summarization and Clustering

Ausejo-Marcos, Raquel (Universidad de Zaragoza) ; Tejedor, María Teresa (Universidad de Zaragoza) ; Miguel-Jiménez, Sara ; Gómez-Giménez, Belén ; Soriano-Úbeda, Cristina ; Mendoza, Noelia ; Vicente-Carrillo, Alejandro ; Hurtado, William Fernando ; Ávila Holguín, Celia ; Moreno, Bernardino (Universidad de Zaragoza) ; Falceto, María Victoria (Universidad de Zaragoza)

Resumen: Boar semen analysis includes sperm motility, concentration, morphology and other more complex analyses such as membrane integrity, DNA damage and seminal plasma components. This study aims to summarize these numerous data by linear combinations of them, to classify ejaculates in several categories (clusters) and to investigate the potential differences among clusters on fertility and prolificacy. Young Pietrain boars (23 ± 3.6 months) were investigated: ten boars from the Nucléus genetic line (group 1: 90 ejaculates weekly) and five boars from the Batallé genetic line (group 2: 30 ejaculates weekly). Computer-assisted semen analysis (CASA) examined motility. Sperm viability, acrosome reaction, early apoptosis, mitochondrial activity and DNA damage were studied by flow cytometry analysis. SPSS v.26 software was used to perform principal component analysis (PCA) and clustering. Three principal components (PC1: speed; PC2: linear path; PC3: DNA damage) were detected and four clusters identified in both groups. Clusters also differed significantly in several variables not included in these PCs (group 1: beat cross frequency and poly (ADP-ribose) polymerase; group 2: cathepsin B, abnormal forms, mitochondrial activity and high DNA stainability). PCA and clustering achieved adequate description of these ejaculates, but no differences among clusters were found for fertility or prolificacy, probably because the minimum sperm requirements had been met.

Idioma: Inglés

DOI: 10.3390/vetsci11090420

Año: 2024

Publicado en: Veterinary Sciences 11, 9 (2024), 420 [17 pp.]
ISSN: 2306-7381

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)