000145751 001__ 145751
000145751 005__ 20241122130824.0
000145751 0247_ $$2doi$$a10.1016/j.microc.2024.112013
000145751 0248_ $$2sideral$$a140610
000145751 037__ $$aART-2024-140610
000145751 041__ $$aeng
000145751 100__ $$aDejonghe, Rinus
000145751 245__ $$aAn evaluation of the analytical and biological robustness of a method for quantifying iron in individual red blood cells via single-cell tandem ICP-mass spectrometry
000145751 260__ $$c2024
000145751 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145751 5203_ $$aThis work evaluated the analytical and biological robustness of iron (Fe) determination in individual red blood cells (RBCs) via single-cell ICP-MS (SC-ICP-MS). RBCs were separated from other whole blood constituents using Ficoll-Paque™ density gradient centrifugation. While fixation with paraformaldehyde (PFA) led to RBC lysis, the use of glutaraldehyde (GA) left the RBCs intact and permitted storage of RBC suspensions in ultra-pure water for up to 16 months at 4 °C. GA-fixation also rendered the RBCs sufficiently robust to maintain integrity during their introduction into the ICP by means of nebulization of dilute suspensions. Obtaining quantitative data on a cell-per-cell basis required determination of the transport efficiency using the particle size method and external calibration against aqueous Fe standard solutions. The average Fe content per RBC obtained using SC-ICP-MS (103 fg/cell) agreed well with the value obtained using solution-based ICP-MS obtained after cell pellet digestion and with values obtained from literature. Variation of the cell number density in the suspensions analyzed between 1.5 × 105 and 6.0 × 105 cells per mL did not affect the result. Identical results from one-week interval blood drawings from healthy individuals demonstrate biological consistency. Compared to bulk analysis, the SC-ICP-MS approach offers the added value of providing information on the cell-to-cell heterogeneity in Fe content.
000145751 536__ $$9info:eu-repo/grantAgreement/EC/H2020/101034288/EU/International Fellowship Programme for Talent Attraction to the Campus of International Excellence Campus Iberus/IberusExperience$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101034288-IberusExperience$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2021-031093-I
000145751 540__ $$9info:eu-repo/semantics/embargoedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000145751 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000145751 700__ $$0(orcid)0000-0002-1856-2058$$aBolea-Fernández, Eduardo$$uUniversidad de Zaragoza
000145751 700__ $$aLores-Padin, Ana
000145751 700__ $$aVan Acker, Thibaut
000145751 700__ $$0(orcid)0000-0001-9582-6283$$aRua-Ibarz, Ana$$uUniversidad de Zaragoza
000145751 700__ $$aDe Wever, Olivier
000145751 700__ $$aVanhaecke, Frank
000145751 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000145751 773__ $$g207 (2024), 112013 [8 p.]$$pMicrochem. j.$$tMICROCHEMICAL JOURNAL$$x0026-265X
000145751 8564_ $$s2038885$$uhttps://zaguan.unizar.es/record/145751/files/texto_completo.pdf$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2026-10-23
000145751 8564_ $$s1367261$$uhttps://zaguan.unizar.es/record/145751/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2026-10-23
000145751 909CO $$ooai:zaguan.unizar.es:145751$$particulos$$pdriver
000145751 951__ $$a2024-11-22-11:53:48
000145751 980__ $$aARTICLE