145751 20260217205610.0 doi 10.1016/j.microc.2024.112013 sideral 140610 ART-2024-140610 eng Dejonghe, Rinus An evaluation of the analytical and biological robustness of a method for quantifying iron in individual red blood cells via single-cell tandem ICP-mass spectrometry 2024 Access copy available to the general public Unrestricted This work evaluated the analytical and biological robustness of iron (Fe) determination in individual red blood cells (RBCs) via single-cell ICP-MS (SC-ICP-MS). RBCs were separated from other whole blood constituents using Ficoll-Paque™ density gradient centrifugation. While fixation with paraformaldehyde (PFA) led to RBC lysis, the use of glutaraldehyde (GA) left the RBCs intact and permitted storage of RBC suspensions in ultra-pure water for up to 16 months at 4 °C. GA-fixation also rendered the RBCs sufficiently robust to maintain integrity during their introduction into the ICP by means of nebulization of dilute suspensions. Obtaining quantitative data on a cell-per-cell basis required determination of the transport efficiency using the particle size method and external calibration against aqueous Fe standard solutions. The average Fe content per RBC obtained using SC-ICP-MS (103 fg/cell) agreed well with the value obtained using solution-based ICP-MS obtained after cell pellet digestion and with values obtained from literature. Variation of the cell number density in the suspensions analyzed between 1.5 × 105 and 6.0 × 105 cells per mL did not affect the result. Identical results from one-week interval blood drawings from healthy individuals demonstrate biological consistency. Compared to bulk analysis, the SC-ICP-MS approach offers the added value of providing information on the cell-to-cell heterogeneity in Fe content. info:eu-repo/grantAgreement/EC/H2020/101034288/EU/International Fellowship Programme for Talent Attraction to the Campus of International Excellence Campus Iberus/IberusExperience This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101034288-IberusExperience info:eu-repo/grantAgreement/ES/MICINN/RYC2021-031093-I info:eu-repo/semantics/embargoedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 5.1 2024 CHEMISTRY, ANALYTICAL 23 / 111 = 0.207 2024 Q1 T1 0.75 2024 Spectroscopy 2024 Q1 Analytical Chemistry 2024 Q1 7.2 2024 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Bolea-Fernández, Eduardo Universidad de Zaragoza (orcid)0000-0002-1856-2058 Lores-Padin, Ana Van Acker, Thibaut Rua-Ibarz, Ana Universidad de Zaragoza (orcid)0000-0001-9582-6283 De Wever, Olivier Vanhaecke, Frank 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 207 (2024), 112013 [8 p.] Microchem. j. MICROCHEMICAL JOURNAL 0026-265X 2038885 http://zaguan.unizar.es/record/145751/files/texto_completo.pdf Postprint info:eu-repo/date/embargoEnd/2026-10-23 1367261 http://zaguan.unizar.es/record/145751/files/texto_completo.jpg?subformat=icon icon Postprint info:eu-repo/date/embargoEnd/2026-10-23 oai:zaguan.unizar.es:145751 articulos driver 2026-02-17-20:48:45 ARTICLE