145753 20260217205610.0 doi 10.3390/foods13213434 sideral 140634 ART-2024-140634 eng Freire, Víctor Universidad de Zaragoza (orcid)0000-0003-1979-363X Influence of Sporulation Temperature on Germination and Growth of B. weihenstephanensis Strains in Specific Nutrients and in an Extended Shelf-Life Refrigerated Matrix Under Commercial Pasteurization and Storage Conditions 2024 Access copy available to the general public Unrestricted Extended shelf-life (ESL) refrigerated ready-to-eat foods are thermally pasteurized to ensure food safety and stability. However, surviving psychrotrophic Bacillus cereus spores can still pose a challenge. Studies predicting their behavior often overlook sporulation conditions. This study investigated the effect of sporulation temperature on germination of three Bacillus weihenstephanensis strains in specific nutrients (inosine and/or amino acids) with or without prior heat activation (80 °C, 10 min). Sporulation temperature variably affected germination, with stronger effects in moderately responsive strains and nutrients. Heat activation strongly stimulated germination, particularly in nutrients with poorer responses, mitigating differences induced by sporulation temperature. The influence of sporulation temperature on germination and growth in an ESL matrix at refrigeration temperatures (4 °C or 8 °C) in vacuum packaging after heat activation or commercial pasteurization (90 °C, 10 min) was also studied. The latter treatment increased germination rates of surviving spores; however, some strains suffered damage and lost viability upon germination at 4 °C but recovered and grew at 8 °C. These findings highlight the need to account for variability in spore recovery and outgrowth during quantitative risk assessments for psychrotrophic B. cereus in ESL foods. info:eu-repo/grantAgreement/ES/MICINN/PID2019-104712RA-I00 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 5.1 2024 FOOD SCIENCE & TECHNOLOGY 43 / 182 = 0.236 2024 Q1 T1 1.021 2024 Food Science 2024 Q1 Health (social science) 2024 Q1 Plant Science 2024 Q1 Health Professions (miscellaneous) 2024 Q1 Microbiology 2024 Q2 8.7 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Casañas, Lina Universidad de Zaragoza Laborda, Luis Condón, Santiago Universidad de Zaragoza (orcid)0000-0002-1902-0648 Gayán, Elisa Universidad de Zaragoza (orcid)0000-0002-5895-2157 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 13, 21 (2024), 3434 [23 pp.] Foods Foods 2304-8158 2087202 http://zaguan.unizar.es/record/145753/files/texto_completo.pdf Versión publicada 2687517 http://zaguan.unizar.es/record/145753/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:145753 articulos driver 2026-02-17-20:48:47 ARTICLE