000146926 001__ 146926
000146926 005__ 20260112133311.0
000146926 0247_ $$2doi$$a10.1038/s41598-024-79573-y
000146926 0248_ $$2sideral$$a140705
000146926 037__ $$aART-2024-140705
000146926 041__ $$aeng
000146926 100__ $$0(orcid)0000-0003-1599-8216$$aLanda, Guillermo
000146926 245__ $$aImpact of mixed Staphylococcus aureus-Pseudomonas aeruginosa biofilm on susceptibility to antimicrobial treatments in a 3D in vitro model
000146926 260__ $$c2024
000146926 5060_ $$aAccess copy available to the general public$$fUnrestricted
000146926 5203_ $$aStaphylococcus aureus and Pseudomonas aeruginosa are the most common bacteria co-isolated from chronic infected wounds. Their interactions remain unclear but this coexistence is beneficial for both bacteria and may lead to resistance to antimicrobial treatments. Besides, developing an in vitro model where this coexistence is recreated remains challenging, making difficult their study. The aim of this work was to develop a reliable polymicrobial in vitro model of both species to further understand their interrelationships and the effects of different antimicrobials in coculture. In this work, bioluminescent and fluorescent bacteria were used to evaluate the activity of two antiseptics (chlorhexidine and thymol) against these bacteria planktonically grown, or when forming single and mixed biofilms. At the doses tested (0.4-1,000 mg/L), thymol showed selective antimicrobial action against S. aureus in planktonic and biofilm states, in contrast with chlorhexidine which exerted antimicrobial effects against both bacteria. Furthermore, the initial conditions for both bacteria in the co-culture determined the antimicrobial outcome, showing that P. aeruginosa impaired the proliferation and metabolism of S. aureus. Moreover, S. aureus showed an increased tolerance against antiseptic treatments when co-cultured, attributed to the formation of a thicker mixed biofilm compared to those obtained when monocultured, and also, by the reduction of S. aureus metabolic activity induced by diffusible molecules produced by P. aeruginosa. This work underlines the relevance of polymicrobial populations and their crosstalk and microenvironment in the search of disruptive and effective treatments for polymicrobial biofilms.
000146926 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/MS19-00092$$9info:eu-repo/grantAgreement/ES/MCINN/PRE2018-085769$$9info:eu-repo/grantAgreement/ES/MICINN/PDC2021-121405-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-113987RB-I00
000146926 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000146926 590__ $$a3.9$$b2024
000146926 592__ $$a0.874$$b2024
000146926 591__ $$aMULTIDISCIPLINARY SCIENCES$$b25 / 135 = 0.185$$c2024$$dQ1$$eT1
000146926 593__ $$aMultidisciplinary$$c2024$$dQ1
000146926 594__ $$a6.7$$b2024
000146926 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000146926 700__ $$aClarhaut, Jonathan
000146926 700__ $$aBuyck, Julien
000146926 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia
000146926 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000146926 700__ $$aTewes, Frederic
000146926 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000146926 773__ $$g14, 1 (2024), 27877 [14 pp.]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322
000146926 8564_ $$s3162081$$uhttps://zaguan.unizar.es/record/146926/files/texto_completo.pdf$$yVersión publicada
000146926 8564_ $$s2403951$$uhttps://zaguan.unizar.es/record/146926/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000146926 909CO $$ooai:zaguan.unizar.es:146926$$particulos$$pdriver
000146926 951__ $$a2026-01-12-13:02:09
000146926 980__ $$aARTICLE