000147009 001__ 147009
000147009 005__ 20241205091043.0
000147009 0247_ $$2doi$$a10.1016/j.cstres.2024.11.002
000147009 0248_ $$2sideral$$a140857
000147009 037__ $$aART-2024-140857
000147009 041__ $$aeng
000147009 100__ $$aFerrer, Miguel$$uUniversidad de Zaragoza
000147009 245__ $$aProtective role of short-chain fatty acids on intestinal oxidative stress induced by TNF-a
000147009 260__ $$c2024
000147009 5060_ $$aAccess copy available to the general public$$fUnrestricted
000147009 5203_ $$aInflammatory bowel diseases (IBDs) are driven by an exaggerated inflammatory response, which leads to a marked increase in oxidative stress. This, in turn, exacerbates the inflammatory process and causes significant cellular and tissue damage. Intestinal dysbiosis, a common observation in IBD patients, alters the production of bacterial metabolites, including short-chain fatty acids (SCFAs), which are key by-products of dietary fiber fermentation. While the role of SCFAs in intestinal physiology is still being elucidated, this study aimed to investigate their effects on intestinal oxidative stress, particularly under inflammatory conditions induced by the proinflammatory mediator tumor necrosis factor alpha (TNF-α). The Caco-2/TC7 cell line was employed as an in vitro model of the intestinal epithelium, and the cells were treated with a range of SCFAs, including acetate, propionate, and butyrate. The levels of protein and lipid oxidation were quantified, as well as the activity of antioxidant enzymes. Our findings demonstrate that microbiota-derived SCFAs can effectively mitigate TNF-α-induced oxidative stress by modulating antioxidant enzyme activity. The proinflammatory mediator TNF-α induces lipid peroxidation by inhibiting catalase and glutathione peroxidase activities. SCFAs are able to upregulate antioxidant enzyme activity to restore lipid oxidative levels. These results underscore the critical role of the gut microbiota in maintaining intestinal homeostasis and highlight the therapeutic potential of SCFAs in managing oxidative stress-related pathologies.
000147009 536__ $$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2018-BIO-04
000147009 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000147009 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000147009 700__ $$0(orcid)0000-0002-8467-0356$$aBuey, Berta$$uUniversidad de Zaragoza
000147009 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa, Laura$$uUniversidad de Zaragoza
000147009 700__ $$0(orcid)0000-0003-4758-3998$$aMesonero, Jose Emilio$$uUniversidad de Zaragoza
000147009 700__ $$0(orcid)0000-0002-5797-3909$$aLatorre, Eva$$uUniversidad de Zaragoza
000147009 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000147009 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000147009 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000147009 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000147009 773__ $$g29, 6 (2024), 769-776$$pCell stress chaperones$$tCELL STRESS & CHAPERONES$$x1355-8145
000147009 8564_ $$s594470$$uhttps://zaguan.unizar.es/record/147009/files/texto_completo.pdf$$yVersión publicada
000147009 8564_ $$s2563402$$uhttps://zaguan.unizar.es/record/147009/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000147009 909CO $$ooai:zaguan.unizar.es:147009$$particulos$$pdriver
000147009 951__ $$a2024-12-05-08:46:34
000147009 980__ $$aARTICLE