000147039 001__ 147039 000147039 005__ 20241205091043.0 000147039 0247_ $$2doi$$a10.1016/j.bpj.2024.08.003 000147039 0248_ $$2sideral$$a140402 000147039 037__ $$aART-2024-140402 000147039 041__ $$aeng 000147039 100__ $$0(orcid)0000-0002-9361-4794$$aDe Corato, Marco$$uUniversidad de Zaragoza 000147039 245__ $$aInterplay of chromatin organization and mechanics of the cell nucleus 000147039 260__ $$c2024 000147039 5203_ $$aThe nucleus of eukaryotic cells is constantly subjected to different kinds of mechanical stimuli, which can impact the organization of chromatin and, subsequently, the expression of genetic information. Experiments from different groups showed that nuclear deformation can lead to transient or permanent condensation or decondensation of chromatin and the mechanical activation of genes, thus altering the transcription of proteins. Changes in chromatin organization, in turn, change the mechanical properties of the nucleus, possibly leading to an auxetic behavior. Here, we model the mechanics of the nucleus as a chemically active polymer gel in which the chromatin can exist in two states: a self-attractive state representing the heterochromatin and a repulsive state representing euchromatin. The model predicts reversible or irreversible changes in chromatin condensation levels upon external deformations of the nucleus. We find an auxetic response for a broad range of parameters under small and large deformations. These results agree with experimental observations and highlight the key role of chromatin organization in the mechanical response of the nucleus. 000147039 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-124271OB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2021-030948-I 000147039 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000147039 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000147039 700__ $$0(orcid)0000-0002-1878-8997$$aGomez-Benito, Maria Jose$$uUniversidad de Zaragoza 000147039 7102_ $$15001$$2600$$aUniversidad de Zaragoza$$bDpto. Ciencia Tecnol.Mater.Fl.$$cÁrea Mecánica de Fluidos 000147039 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est. 000147039 773__ $$g123, 19 (2024), 3386-3396$$pBiophys. j.$$tBIOPHYSICAL JOURNAL$$x0006-3495 000147039 8564_ $$s2994066$$uhttps://zaguan.unizar.es/record/147039/files/texto_completo.pdf$$yVersión publicada 000147039 8564_ $$s2673358$$uhttps://zaguan.unizar.es/record/147039/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000147039 909CO $$ooai:zaguan.unizar.es:147039$$particulos$$pdriver 000147039 951__ $$a2024-12-05-08:47:05 000147039 980__ $$aARTICLE