000147103 001__ 147103
000147103 005__ 20250923084439.0
000147103 0247_ $$2doi$$a10.1007/s00253-024-13314-z
000147103 0248_ $$2sideral$$a140904
000147103 037__ $$aART-2024-140904
000147103 041__ $$aeng
000147103 100__ $$aCinca-Fernando, Paula$$uUniversidad de Zaragoza
000147103 245__ $$aDiscovery, characterization, and synthetic potential of two novel bacterial aryl-alcohol oxidases
000147103 260__ $$c2024
000147103 5060_ $$aAccess copy available to the general public$$fUnrestricted
000147103 5203_ $$aThe search for novel synthetic tools to prepare industrial chemicals in a safer and greener manner is a continuing challenge in synthetic chemistry. In this manuscript, we report the discovery, characterization, and synthetic potential of two novel aryl-alcohol oxidases from bacteria which are able to oxidize a variety of aliphatic and aromatic alcohols with efficiencies up to 4970 min−1 mM−1. Both enzymes have shown a reasonable thermostability (thermal melting temperature values of 50.9 and 48.6 °C for ShAAO and SdAAO, respectively). Crystal structures revealed an unusual wide-open entrance to the active-site pockets compared to that previously described for traditional fungal aryl-alcohol oxidases, which could be associated with differences observed in substrate scope, catalytic efficiency, and other functional properties. Preparative-scale reactions and the ability to operate at high substrate loadings also demonstrate the potential of these enzymes in synthetic chemistry with total turnover numbers > 38000. Moreover, their availability as soluble and active recombinant proteins enabled their use as cell-free extracts which further highlights their potential for the large-scale production of carbonyl compounds.
Key points
• Identification and characterization of two novel bacterial aryl-alcohol oxidases
• Crystal structures reveal wide-open active-site pockets, impacting substrate scope
• Total turnover numbers and cell-free extracts demonstrate the synthetic potential
000147103 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-136369NB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2021-032021-I$$9info:eu-repo/grantAgreement/EUR/MICINN/TED2021-130803B-I00
000147103 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000147103 590__ $$a4.3$$b2024
000147103 592__ $$a0.967$$b2024
000147103 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b42 / 177 = 0.237$$c2024$$dQ1$$eT1
000147103 593__ $$aApplied Microbiology and Biotechnology$$c2024$$dQ1
000147103 593__ $$aMedicine (miscellaneous)$$c2024$$dQ1
000147103 593__ $$aBiotechnology$$c2024$$dQ2
000147103 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000147103 700__ $$aAscaso-Alegre, Christian$$uUniversidad de Zaragoza
000147103 700__ $$0(orcid)0000-0001-6435-3540$$aSevilla, Emma$$uUniversidad de Zaragoza
000147103 700__ $$0(orcid)0000-0001-9047-0046$$aMartínez-Júlvez, Marta$$uUniversidad de Zaragoza
000147103 700__ $$aMangas-Sánchez, Juan
000147103 700__ $$0(orcid)0000-0003-4076-6118$$aFerreira, Patricia$$uUniversidad de Zaragoza
000147103 7102_ $$11002$$2412$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Fisiología Vegetal
000147103 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000147103 773__ $$g108, 1 (2024)$$pAppl. microbiol. biotechnol.$$tAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY$$x0175-7598
000147103 8564_ $$s1924609$$uhttps://zaguan.unizar.es/record/147103/files/texto_completo.pdf$$yVersión publicada
000147103 8564_ $$s2264516$$uhttps://zaguan.unizar.es/record/147103/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000147103 909CO $$ooai:zaguan.unizar.es:147103$$particulos$$pdriver
000147103 951__ $$a2025-09-22-14:49:00
000147103 980__ $$aARTICLE