000147110 001__ 147110
000147110 005__ 20241212141912.0
000147110 0247_ $$2doi$$a10.1038/s41598-024-80931-z
000147110 0248_ $$2sideral$$a140932
000147110 037__ $$aART-2024-140932
000147110 041__ $$aeng
000147110 100__ $$aBaselga, Marta
000147110 245__ $$aPreclinical evaluation of polymer encapsulated carbon-based nano and microparticles for sentinel lymph node tattooing
000147110 260__ $$c2024
000147110 5060_ $$aAccess copy available to the general public$$fUnrestricted
000147110 5203_ $$aSelective sentinel lymph node biopsy (SNLB) is the standard method for detecting regional metastases in breast cancer patients. Identifying affected axillary lymph nodes before neoadjuvant treatment is crucial, as such treatment may alter drainage pathways and lymph node morphology, hindering the identification of sentinel lymph nodes. The use of carbon-based tattooing on sentinel lymph nodes (SLN) has been employed as a permanent tattooing method in clinical studies of Targeted Axillary Dissection (TAD), aiding in the SLN identification during surgery. Our study introduces a new method of lymph node tattooing based on poly lactic-co-glycolic (PLGA) particles with encapsulated carbon. This strategy substantially improves tattooing efficiency over single carbon suspensions currently used in clinical studies. We synthesized and characterized carbon-loaded PLGA micro- and nanoparticles, experimentally assessing their biological impact on porcine lymph nodes. The effect of particles’ size and concentration was evaluated over time (from 1 to 16 weeks). Light and electron microscopy studies were conducted to characterize the cellular effects induced by the presence of these particles. Our findings reveal that the diverse physicochemical parameters of the particles interact differently with the lymphatic tissue, influencing their biodistribution within the lymph nodes and the intensity of the inflammatory response.
000147110 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/DTS20-00131
000147110 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000147110 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000147110 700__ $$0(orcid)0000-0002-4437-2581$$aGüemes, Antonio$$uUniversidad de Zaragoza
000147110 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000147110 700__ $$0(orcid)0000-0002-1505-498X$$aYus, Cristina$$uUniversidad de Zaragoza
000147110 700__ $$0(orcid)0000-0002-9324-0446$$aAlejo, Teresa
000147110 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, Víctor$$uUniversidad de Zaragoza
000147110 700__ $$0(orcid)0000-0001-7872-6301$$aMartínez, Gema
000147110 700__ $$0(orcid)0000-0002-3592-820X$$aArribas, Dolores$$uUniversidad de Zaragoza
000147110 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia
000147110 700__ $$0(orcid)0000-0002-9951-1075$$aJunquera, Concepción$$uUniversidad de Zaragoza
000147110 700__ $$0(orcid)0000-0002-7453-1766$$aMonleón, Eva$$uUniversidad de Zaragoza
000147110 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000147110 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000147110 7102_ $$11013$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Cirugía
000147110 773__ $$g14, 1 (2024), 29512 [17 pp.]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322
000147110 8564_ $$s4097881$$uhttps://zaguan.unizar.es/record/147110/files/texto_completo.pdf$$yVersión publicada
000147110 8564_ $$s2015435$$uhttps://zaguan.unizar.es/record/147110/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000147110 909CO $$ooai:zaguan.unizar.es:147110$$particulos$$pdriver
000147110 951__ $$a2024-12-12-12:43:09
000147110 980__ $$aARTICLE