147110 20260217205534.0 doi 10.1038/s41598-024-80931-z sideral 140932 ART-2024-140932 eng Baselga, Marta (orcid)0000-0003-2672-5790 Preclinical evaluation of polymer encapsulated carbon-based nano and microparticles for sentinel lymph node tattooing 2024 Access copy available to the general public Unrestricted Selective sentinel lymph node biopsy (SNLB) is the standard method for detecting regional metastases in breast cancer patients. Identifying affected axillary lymph nodes before neoadjuvant treatment is crucial, as such treatment may alter drainage pathways and lymph node morphology, hindering the identification of sentinel lymph nodes. The use of carbon-based tattooing on sentinel lymph nodes (SLN) has been employed as a permanent tattooing method in clinical studies of Targeted Axillary Dissection (TAD), aiding in the SLN identification during surgery. Our study introduces a new method of lymph node tattooing based on poly lactic-co-glycolic (PLGA) particles with encapsulated carbon. This strategy substantially improves tattooing efficiency over single carbon suspensions currently used in clinical studies. We synthesized and characterized carbon-loaded PLGA micro- and nanoparticles, experimentally assessing their biological impact on porcine lymph nodes. The effect of particles’ size and concentration was evaluated over time (from 1 to 16 weeks). Light and electron microscopy studies were conducted to characterize the cellular effects induced by the presence of these particles. Our findings reveal that the diverse physicochemical parameters of the particles interact differently with the lymphatic tissue, influencing their biodistribution within the lymph nodes and the intensity of the inflammatory response. info:eu-repo/grantAgreement/ES/ISCIII/DTS20-00131 info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 3.9 2024 MULTIDISCIPLINARY SCIENCES 25 / 136 = 0.184 2024 Q1 T1 0.874 2024 Multidisciplinary 2024 Q1 6.7 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Güemes, Antonio Universidad de Zaragoza (orcid)0000-0002-4437-2581 Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Yus, Cristina Universidad de Zaragoza (orcid)0000-0002-1505-498X Alejo, Teresa (orcid)0000-0002-9324-0446 Sebastián, Víctor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Martínez, Gema (orcid)0000-0001-7872-6301 Arribas, Dolores Universidad de Zaragoza (orcid)0000-0002-3592-820X Mendoza, Gracia (orcid)0000-0003-2293-363X Junquera, Concepción Universidad de Zaragoza (orcid)0000-0002-9951-1075 Monleón, Eva Universidad de Zaragoza (orcid)0000-0002-7453-1766 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 1013 090 Universidad de Zaragoza Dpto. Cirugía Área Cirugía 14, 1 (2024), 29512 [17 pp.] Sci. rep. (Nat. Publ. Group) Scientific reports (Nature Publishing Group) 2045-2322 4097881 http://zaguan.unizar.es/record/147110/files/texto_completo.pdf Versión publicada 2015435 http://zaguan.unizar.es/record/147110/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:147110 articulos driver 2026-02-17-20:33:34 ARTICLE