147178 20260217205606.0 doi 10.1080/01652176.2024.2424837 sideral 140948 ART-2024-140948 eng Pérez-Lázaro, Sonia Universidad de Zaragoza (orcid)0000-0002-2079-0711 New preclinical biomarkers for prion diseases in the cerebrospinal fluid proteome revealed by mass spectrometry 2024 Access copy available to the general public Unrestricted Current diagnostic methods for prion diseases only work in late stages of the disease when neurodegeneration is irreversible. Therefore, biomarkers that can detect the disease before the onset of clinical symptoms are necessary. High-throughput discovery proteomics is of great interest in the search for such molecules. Here we used mass spectrometry to analyse the cerebrospinal fluid proteome in an animal prion disease: preclinical and clinical sheep affected with natural scrapie, and healthy sheep. Interestingly, we found 46 proteins in the preclinical stage that were significantly altered (p < 0.01) compared to healthy sheep, mainly associated with biological processes such as stress and inflammatory responses. Five of them were selected for validation by enzyme-like immunosorbent assay: synaptotagmin binding, cytoplasmic RNA interacting protein (SYNCRIP), involved in nucleic acid metabolism; phospholipase D3 (PLD3) and cathepsin D (CTSD), both related to lysosomal apoptosis; complement component 4 (C4), an element of the classical immune response; and osteopontin (SPP1), a proinflammatory cytokine. These proteins significantly increased in the preclinical stage and maintained their levels in the clinical phase, except for CTSD, whose concentration returned to basal levels in the clinical group. Further research is ongoing to explore their potential as preclinical biomarkers of prion diseases. info:eu-repo/grantAgreement/ES/MICINN/PID2021-125398OB-I00 info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00 info:eu-repo/semantics/openAccess by-nc https://creativecommons.org/licenses/by-nc/4.0/deed.es 5.2 2024 VETERINARY SCIENCES 5 / 170 = 0.029 2024 Q1 T1 1.308 2024 Veterinary (miscellaneous) 2024 Q1 Medicine (miscellaneous) 2024 Q1 7.8 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Barrio, Tomás Bravo, Susana B. Sevilla, Eloisa Universidad de Zaragoza (orcid)0000-0002-4213-2904 Otero, Alicia Universidad de Zaragoza (orcid)0000-0001-9075-2764 Chantada-Vázquez, María del Pilar Martín-Burriel, Inmaculada Universidad de Zaragoza (orcid)0000-0001-6016-4726 Requena, Jesús R. Badiola, Juan J. Universidad de Zaragoza (orcid)0000-0002-7173-7216 Bolea, Rosa Universidad de Zaragoza (orcid)0000-0002-2746-3932 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 44, 1 (2024), 1-15 Vet. q. VETERINARY QUARTERLY 0165-2176 ProteomeXchange Consortium - Mass spectrometry proteomic data PXD050656 2193486 http://zaguan.unizar.es/record/147178/files/texto_completo.pdf Versión publicada 3096801 http://zaguan.unizar.es/record/147178/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:147178 articulos driver 2026-02-17-20:47:10 ARTICLE