000147186 001__ 147186
000147186 005__ 20260112133357.0
000147186 0247_ $$2doi$$a10.1016/j.ijpharm.2024.124951
000147186 0248_ $$2sideral$$a140903
000147186 037__ $$aART-2024-140903
000147186 041__ $$aeng
000147186 100__ $$0(orcid)0000-0002-1505-498X$$aYus, Cristina$$uUniversidad de Zaragoza
000147186 245__ $$aDevelopment of a hybrid CuS-ICG polymeric photosensitive vector and its application in antibacterial photodynamic therapy
000147186 260__ $$c2024
000147186 5060_ $$aAccess copy available to the general public$$fUnrestricted
000147186 5203_ $$aAt the present time, owing to the extremely high growth of microbial resistance to antibiotics and, consequently, the increased healthcare associated costs and the loss of efficacy of current treatments, the development of new therapies against bacteria is of paramount importance. For this reason, in this work, a hybrid synergetic nanovector has been developed, based on the encapsulation of a NIR (near infrared) photosensitive molecule (indocyanine green, ICG) in biodegradable polymeric nanoparticles (NPs). In addition, copper sulfide nanoparticles (CuS NPs), optically sensitive to NIR, were anchored on the polymeric nanoparticle shell in order to boost the generation of reactive oxygen species (ROS) upon NIR irradiation. As a result, the nanohybrid synthesized material is capable to generate ROS on demand when exposed to a NIR laser (808 nm) allowing for the repeated triggering of ROS production upon NIR light exposure. After each irradiation, the ROS generated were able to eliminate pathogenic bacteria, as it was demonstrated in-vitro with three bacterial strains, Staphylococcus aureus ATCC 25923 used as a reference strain (S. aureus), S. aureus USA300 (methicillin-resistantstrain, MRSA) and GFP-expressing antibiotic-sensitive S. aureus (methicillin-sensitive strain, MSSA). Finally, the effect of the hybrid NPs in the skin bed was tested on a plasma-derived in vitro skin model. Fluorescence and histological images showed the presence of CuS NPs all over the dermal layer lacking epidermis of the skin construct. Thus, the in vitro model facilitated the prediction of the nanovector's behavior in a human skin equivalent, showcasing its potential application against topical infections after wounding.
000147186 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII-FEDER/CIBER-BBN$$9info:eu-repo/grantAgreement/ES/MICINN/PDC2021-121405-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PDC2022-133866-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-113987RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-127847OB-I00$$9info:eu-repo/grantAgreement/ES/UZ/LMA-ICTS ELECMI-CIBER-BBN
000147186 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000147186 590__ $$a5.2$$b2024
000147186 592__ $$a0.988$$b2024
000147186 591__ $$aPHARMACOLOGY & PHARMACY$$b43 / 352 = 0.122$$c2024$$dQ1$$eT1
000147186 593__ $$aPharmaceutical Science$$c2024$$dQ1
000147186 594__ $$a10.1$$b2024
000147186 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000147186 700__ $$0(orcid)0000-0002-9324-0446$$aAlejo, Teresa
000147186 700__ $$aQuílez, Cristina
000147186 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta, Silvia
000147186 700__ $$aVelasco, Diego
000147186 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000147186 700__ $$0(orcid)0000-0002-6873-5244$$aSebastian, Victor$$uUniversidad de Zaragoza
000147186 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000147186 773__ $$g667 (2024), 124951 [14 pp.]$$pInt. j. pharm.$$tInternational Journal of Pharmaceutics$$x0378-5173
000147186 8564_ $$s9608971$$uhttps://zaguan.unizar.es/record/147186/files/texto_completo.pdf$$yVersión publicada
000147186 8564_ $$s2497534$$uhttps://zaguan.unizar.es/record/147186/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000147186 909CO $$ooai:zaguan.unizar.es:147186$$particulos$$pdriver
000147186 951__ $$a2026-01-12-13:22:03
000147186 980__ $$aARTICLE